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IJSTR >> Volume 9 - Issue 1, January 2020 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Fucoidan Extract Induces Apoptosis In MCF-7 Cell Line Via Bcl2 And Caspase3 Assay In An In-Vitro Condition

[Full Text]

 

AUTHOR(S)

Revathi Chitra Santhanam, Syed Ali Mohammed Yahoob, Anuradha Venkatraman.

 

KEYWORDS

Apoptosis, Bcl2, Caspase3 assay, MCF7, Programmed cell death.

 

ABSTRACT

Fucoidan extract (FE) is a type of sulphated polysaccharide extracted from Turbinaria conoides, it plays a vital role in treating many severe ailments. In our study, FE extracted from Turbinaria conoides was used for analyzing the induction of apoptotic effect of MCF7 breast cancer cell line using the Bcl2 and Caspase3 assay and it was compared with the standard camptothecin by flow cytometry instrument. From our results it was found that FE induced apoptosis expressions against the MCF7 breast cancer cell line compared to the standard camptothecin. Thus our apoptotic results indicate that FE obtained from the Turbinaia conoides was found to have possible therapeutic activity in invitro condition against breast cancer cell line. It can be further analyzed to confirm its anticancer activity using the invivo studies.

 

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