International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020


IJSTR >> Volume 9 - Issue 1, January 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Fucoidan Extract Induces Apoptosis In MCF-7 Cell Line Via Bcl2 And Caspase3 Assay In An In-Vitro Condition

[Full Text]



Revathi Chitra Santhanam, Syed Ali Mohammed Yahoob, Anuradha Venkatraman.



Apoptosis, Bcl2, Caspase3 assay, MCF7, Programmed cell death.



Fucoidan extract (FE) is a type of sulphated polysaccharide extracted from Turbinaria conoides, it plays a vital role in treating many severe ailments. In our study, FE extracted from Turbinaria conoides was used for analyzing the induction of apoptotic effect of MCF7 breast cancer cell line using the Bcl2 and Caspase3 assay and it was compared with the standard camptothecin by flow cytometry instrument. From our results it was found that FE induced apoptosis expressions against the MCF7 breast cancer cell line compared to the standard camptothecin. Thus our apoptotic results indicate that FE obtained from the Turbinaia conoides was found to have possible therapeutic activity in invitro condition against breast cancer cell line. It can be further analyzed to confirm its anticancer activity using the invivo studies.



[1] Soule HD, Vazguez J, Long A, Albert S, Brennan M A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 51:1409-1416, 1973.
[2] Wang J, Zhang Q, Zhang Z, Li Z (2008) Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int J BiolMacromol. 42: 127–132.
[3] Xiaolin Chena, Ronge Xing, Huahua Yu, Pengcheng li.A new extraction method of Fucoidan from the soaked water of Brown seaweed (Laminaria japonica) Desalination and Water Treatment,2012;40: 204–208.
[4] Revathi Chitra Santhanam, Syed Ali Mohammed Yahoob, Anuradha Venkatraman. Anti Scavenging Property And Characterization Using Hplc Of The Fucoidan Extracted From Turbinaria Conoides. International Journal Of Scientific & Technology Research Volume 8, Issue 11, November 2019 Issn 2277-8616
[5] Meilan Xue1 , Yinlin Ge1, Jinyu Zhang1 , Qing Wang2 , Lin Hou1 , Yongchao Liu1 , Lingling Sun3 , Quan Li1 1 Anticancer Properties and Mechanisms of Fucoidan on Mouse Breast Cancer In Vitro and In Vivo PLoS ONE 7(8): e43483. doi:10.1371/journal.pone.0043483 )2012
[6] Raisova M, Hossini AM, Eberle .J,Riebeling C, Wieder T,et.al.The bax/bcl2 ratio determines the susceptibility of human melanoma cells to CD95/Fas- mediated apoptosis.J Invest Dermatol 117:333-340,2001
[7] Zhongyuan Zhang , Kiichiro Teruya , Hiroshi Eto, Sanetaka Shirahata:FE Induces Apoptosis in MCF-7 Cells via a Mechanism Involving the ROS-Dependent JNK Activation and Mitochondria-Mediated Pathways PLoS/one peer-reviewed open accesshttps://doi.org/10.1371/journal.pone.0027441 Published: November 11, 2011