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IJSTR >> Volume 9 - Issue 2, February 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Biologically Active Fraction Of Flemingia Wightiana's Ethanolic Leaf Extract And Its Cytotoxic Properties

[Full Text]



M. Lakshmi Priya, C. V. Naidu, Josthna Penchalaneni



Flemingia wightiana; Ethanolic leaf extract; Fractions; Bioactive compounds; Cytotoxicity; HCT-116 cancer cell lines; Anti-cancer.



Upon purification, various anti-cancer drugs derived from plant materials were tested on cells, including various cancer cell lines and laboratory animals and then sent to clinical trials. In recent years, the number of newly discovered natural compounds has increased dynamically.Such compounds have cytotoxic properties with a range of action mechanisms such as tumor cell growth inhibition, apoptosis induction, DNA disruption, topoisomerase inhibition I and II, apoptosis induction, and others.Our present study deals with extraction and isolation of different fractions of Flemingia wightiana's ethanolic leaf extract by using column chromatography and thin layer chromatography [TLC]. This purification results in three different fractions where these three were subjected to identify their cytotoxicity against HCT-116 cancer cell lines.MTT reports make it clear that fraction 1 is the active fraction of all, and this is further continued to find the exact bioactive compounds through GC-MS.



[1] Dias, D. A., Urban, S. & Roessner, U. [2012], A historical overview of natural products in drug discovery. Metabolites, 2[2]: 303-336.
[2] Teinkela, J.E.M., Noundou, X.S., Nguemfo, E.L., Meyer, F., Wintjens, R., Isaacs, M., Mpondo, A.E.M., Hoppe, H.C., Krause, R.W.M. and Azebaze, A.G.B. [2018], Biological activities of plant extracts from Ficus elastica and Selaginella vogelli: An antimalarial, antitrypanosomal and cytotoxity evaluation. Saudi journal of biological sciences, 25[1]: 117-122.
[3] Khan, R.A. [2018], Natural products chemistry: The emerging trends and prospective goals. Saudi pharmaceutical journal, 26[5]:739-53.
[4] Newman, D.J.,& Cragg, G.M. [2016], Natural products as sources of new drugs from 1981 to 2014. Journal of natural products, 79[3]:629-61.
[5] Gibson, T.M., Ferrucci, L.M., Tangrea, J.A. & Schatzkin, A. [2010], Epidemiological and clinical studies of nutrition. In: Seminars in oncology,WB Saunders, 37[3]: 282-296.
[6] Kim, H., Caulfield, L.E., Garcia‐Larsen, V., Steffen, L.M., Coresh, J. and Rebholz, C.M. [2019], Plant‐Based Diets Are Associated With a Lower Risk of Incident Cardiovascular Disease, Cardiovascular Disease Mortality, and All‐Cause Mortality in a General Population of Middle‐Aged Adults. Journal of the American Heart Association, 8[16]:e012865.
[7] Greenwell, M.,& Rahman, P.K. [2015], Medicinal plants: their use in anticancer treatment. International journal of pharmaceutical sciences and research, 6[10]:4103.
[8] Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., Linder, T., Wawrosch, C., Uhrin, P., Temml, V., Wang, L., Schwaiger, S., Heiss, E.H. and Rollinger, J.M. [2015], Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology advances, 33[8]:1582-614.
[9] Działo, M., Mierziak, J., Korzun, U., Preisner, M., Szopa, J. and Kulma, A. [2016], The potential of plant phenolics in prevention and therapy of skin disorders. International journal of molecular sciences, 17[2]:160.
[10] Arulselvan, P., Fard, M.T., Tan, W.S., Gothai, S., Fakurazi, S., Norhaizan, M.E. and Kumar, S.S. [2016], Role of antioxidants and natural products in inflammation. Oxidative medicine and cellular longevity, 2016: 5276130.
[11] Pan, S.Y., Zhou, S.F., Gao, S.H., Yu, Z.L., Zhang, S.F., Tang, M.K., Sun, J.N., Ma, D.L., Han, Y.F., Fong, W.F. and Ko, K.M. [2013], New perspectives on how to discover drugs from herbal medicines: CAM's outstanding contribution to modern therapeutics. Evidence-Based Complementary and Alternative Medicine, 2013: 627375.
[12] Patel, K.N., Patel, J.K., Patel, M.P., Rajput, G.C. and Patel, H.A. [2010], Introduction to hyphenated techniques and their applications in pharmacy. Pharmaceutical methods, 1[1] : 2-13.
[13] Jantan, I., Bukhari, S.N.A., Mohamed, M.A.S., Wai, L.K. and Mesaik, M.A. [2015], The evolving role of natural products from the tropical rainforests as a replenishable source of new drug leads. Drug Discovery and Development: From Molecules to Medicine,3-38.
[14] Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G. and Lightfoot, D.A. [2017], Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6 [4] :42.
[15] Liu, Y., Yin, T., Feng, Y., Cona, M.M., Huang, G., Liu, J., Song, S., Jiang, Y., Xia, Q., Swinnen, J.V. and Bormans, G. [2015], Mammalian models of chemically induced primary malignancies exploitable for imaging-based preclinical theragnostic research. Quantitative imaging in medicine and surgery, 5[5] : 708.
[16] Blagosklonny, M.V. [2005] , Carcinogenesis, cancer therapy and chemoprevention. Cell death and differentiation, 12[6]:592.
[17] Cowan, M.M. [1999], Plant products as antimicrobial agents. Clinical microbiology reviews, 12[4]:564-82.
[18] Meybodi, N.M., Mortazavian, A.M., Monfared, A.B., Sohrabvandi, S. and Meybodi, F.A. [2017], Phytochemicals in cancer prevention: a review of the evidence. Iranian Journal of Cancer Prevention, 10[1].
[19] Kaur, V., Kumar, M., Kumar, A., Kaur, K., Dhillon, V.S. and Kaur, S. [2018], Pharmacotherapeutic potential of phytochemicals: Implications in cancer chemoprevention and future perspectives. Biomedicine & Pharmacotherapy, 97: 564-586.
[20] Debiyi, O.O., and Sofowora, F.A. [1978], Pytochemical screening of medical plants. Iloyidia, 3: 234-246.
[21] Roopashree, T.S., Dang, R., Rani, S.R.H. and Narendra, C. [2008], Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied research in Natural products, 1[3] : 20-28.
[22] Trease, G.E., Evans, W.C. [1989], Phenols and phenolic glycosides. In: Textbook of Pharmacognosy, 12 : 343–383, Balliese, Tindall and Co Publishers, London, UK.
[23] Gul, R., Jan, S.U., Faridullah, S., Sherani, S. and Jahan, N. [2017], Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. The Scientific World Journal, 2017.
[24] Eswaraiah, G., Peele, K.A., Krupanidhi, S., Indira, M., Kumar, R.B. and Venkateswarulu, T.C. [2019], GC–MS analysis for compound identification in leaf extract of Lumnitzera racemosa and evaluation of its in vitro anticancer effect against MCF7 and HeLa cell lines. Journal of King Saud University-Science. https://doi.org/10.1016/ j.jksus.2019.01.014.
[25] Bolla, S.R., Al-Subaie, A.M., Al-Jindan, R.Y., Balakrishna, J.P., Ravi, P.K., Veeraraghavan, V.P., Pillai, A.A., Gollapalli, S.S.R., Joseph, J.P. and Surapaneni, K.M. [2019], In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression. Heliyon, 5[5]: e01648.