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IJSTR >> Volume 9 - Issue 2, February 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Effect Of Different C:N Ratio Media On The Spore Production Of Bacillus Firmus

[Full Text]



Kartika Septiana Kusumaningrum, Sri Andayani, Ating Yuniarti



Bacillus, Carbon, C:N Ratio, Aquaculture, Media, Nitrogen, Probiotic, Sporulation.



Bacillus is bacteria which can produce a single spore called endospore. In this recent years a lot of research on Bacillus has been carried out. In aquaculture, bacterial spores can be used as additional ingredients in feed, namely probiotics. Bacillus spore have many advantages compared to vegetative cells. To support the growth of Bacillus spores that have many advantages in aquaculture, this research was using media with glucose as carbon resource and extract yeast as nitrogen source. This works evaluated the effect of different C:N Ratio media on vegetative cells, spore production, and sporulation efficiency of B. firmus. By using media which contain adjustable C:N ratio hopefuly could minimalized the cost of growth medium for B. firmus spores. The result showed that the highest vegetative cells of B. firmus (1,95 x 108 cell/ml) was reach in the media with C:N ratio 11, the highest spore production of B. firmus (1,46 x108 cells/ml) was reach in the media with C:N ratio 5 with 91% sporulation efficiency and it could be used as a potential source of probiotics for aquaculture.



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