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IJSTR >> Volume 2- Issue 2, February 2013 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Ultrastructure, Germination And Viability In Pollens Of Achillea Wilhelmsii C.Koch Exposed To Electromagnetic Fields

[Full Text]

 

AUTHOR(S)

Ahmad Majd, Leila Amjad, Azadeh Ghadirianmarnani

 

KEYWORDS

Index Terms: - electromagnetic field, germination, pollen, viability.

 

ABSTRACT

Abstract: - Electromagnetic field is an inevitable environmental factor for living beings which many investigations have been conducted to consider its effect. In this research the effects of electromagnetic field on pollen ultrastructure, pollen germination and viability of Achillea wilhelmsii were studied. Achillea wilhelmsii C. Koch was collected from around area of Isfahan city (Iran). In case of pollen treatments, the pollens were exposed to electromagnetic field by a magnitude of 2,4 mT at 10 min and 2,4 mT at 20 min in the electromagnetic field generator apparatus. Untreated pollens were used as control under similar condition. Pollens were studied by Scanning electron microscopy. The effect of electromagnetic fields on pollen germination was evaluated. Germination was quatified as the percentage of germinated pollen grains per 100 evaluated. Pollen grains for study of viability, were stained with Evans blue. The results were analyzed using ANOVA statistical to analyses of the treatments. Treatment under pollens with electromagnetic fields 2mT-10,20min have few changes, but treatment under pollens with electromagnetic fields 4mT-20min become abnormal, shrinkage and fragile. the percentage of pollen germination and viability decreased that was significantly(p< 0.05). electromagnetic field have effects on the structure of some organs and developmental characteristic of them in Achillea wilhelmsii. Therefore, a direct influence of environmental stresses is discussed affecting Ca2+ levels via the ion cyclotron resonance mechanism.

 

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TABLE 1
PERCENTAGE GERMINATION AND VIABILITY IN THE CONTROL AND ELECTROMAGNETIC TREATMENT POLLENS
Sample Germination(%) Viability (%)
Control 53.3 51.16
Treatment
2-10 45 46.8
Treatment
2-20 41.17 30.3
Treatment
4-10 31.25 21.87
Treatment
4-10 23.33 12.16

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