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Effectiveness Of Extracted Antibacterial Compound From Hydroid Aglaophenia Cupressina Lamoureoux Against Bacterial Cell Of Escherichia Coli
[Full Text]
AUTHOR(S)
Eva Johannes, Elly Ishak, Hanapi Usman, Mariyati Bilang
KEYWORDS
Keywords: Effectiveness, extracted compound, antibacterial, hydroid Aglaophenia cupressina Lamoureoux, Escherichia coli.
ABSTRACT
Pollution by pathogenic microorganisms in food materials is capable of resulting in various diseases and increased resistance against various types of antimicrobial. Therefore, new more effective antibiotics are needed to overcome the multi-drug resistant bacteria. This study was aimed to examine the effectiveness of extracted antibacterial compound from hydroid Aglaophenia cupressina Lamoureoux in inhibiting and killing the Escherichia coli, an organism that frequently pollute food material. This study was an experimental study with the following treatment steps: isolation and characterization of compounds from hydroin A. cupressina Lamoureoux by chromatography, elucidation of the isolate molecular structure by UV, IR, and NMR. Two isolates obtained were hexadecanoic acid and Agalo E.Unhas, both of them were tested against E. coli at various concentrations (10 ppm, 20 ppm, and 30 ppm). The damage to the E. coli were examined by Electron Microscopic Scanning. Study results indicated that hexadecanoic acid at concentration of 10 ppm and 20 ppm and Aglao E. Unhas at concentration of 10 ppm, 20 ppm, and 30 ppm, was bacteriostatic against E. coli, whereas the hexadecanoic acid at concentration of 30 ppm was bactericidal against E. coli.
REFERENCES
[1]. Suwandi, U., 1993. J. cermin Dunia Kedokteran., 89., 46-48.
[2]. Yuliani, S., 2001. J. Litbang Pertanian Balai Penelitian Tanaman Rempah dan Obat., 20.3.
[3]. Astuti, P., Alam, G.,Pratiwi, S.u.T., Hertiani,T., dan Wahyuono., 2003. J.Biota 8, 127.,47-52
[4]. Ely Rodrigues, Tilvi Supriya, C.G. Naik .,2004. Journal of Experimental Marine Biology and Ecology. 309., 121-127. (www.elsevier.com/locate/jembe ).
[5]. Murniasih, T., 2005. J. Oseana, 3,2, 19-27.
[6]. Joana ; Luisa Peixe ; Newton C.M. Gomes ; Ricardo Calado (2011) . J. Marine Drugs 9, 10, 1860-1886.(www.mdpi.com/journal/marinedrugsReview..Marine Drugs.ISSN 1660-3397).
[7]. Proksch, P., R.A. Edrada and R. Ebel. 2002. Appl. Microbiol. Biotechnol. 59., 124-125.
[8]. Xue Song , Hai Tao Zhang, Pei Chun Wu, Wei Zhang, Quan Yuan., 2004. Journal of Experimental Marine Biology and Ecology. 298.,71– 78. www.elsevier.com/locate/jembe.
[9]. Paradise, M.A. Grassi, G.Conti,F. Passareli and M.G. Curci Abu Erra.,2006. Fire Coral Persistant Cutaneous Reaction (online), (http://jr.science.wep.Muhio,edu/filedcourse)
[10]. Suada, I.K.,and Suniti., 2010., Jurnal Biologi XIV (1).,7-10.
[11]. Johannes., 2008.Isolasi, Karakterisasi dan Uji Bioaktivitas Metyabolit Sekunder dari Hydroid Aglaophenia cupressina Lamoureoux Sebagai Bahan Dasar Antimikroba.(Tesis) Makassar:Universitas Hasanuddin.
[12]. Winarti C. dan Miskiyah, 2010., J. Pengembangan Inovasi Pertanian. 3,3, 227-237.
[13]. BSN (Badan Standarisasi Nasional)., 2009b. Batas Cemaran Mikroba Pada Pangan. BSN. Jakarta.
[14]. Madigan TD. Martinko JM. Parker J. ,2003.Brock Biology of Microorganism.Tenth edition. Pearson Education, Inc.
[15]. Batta Dwi Raj and B.P. Kapadnis., 2010 J.Scientific Word. ,8., 8.
[16]. Kanazawa A. Ikeda T. Endo T., 1995.J ApplBacterial 78., 55-60.
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