Effect Of Chemical Mutation Through Hydroxylamine Hydrochloride On Quantitative Traits Variation In Phaseolus vulgaris L.
Girma Mosisa, Manikandan Muthuswamy, Yohannes Petros
Key words: Induced chemical mutagenesis, haricot bean, hydroxylamine hydrochloride.
Abstract: Chemical mutagenesis is a simple technique used to create mutation in plants for their improvement of their potential agronomic traits. Experiments were conducted in experimental field stations at Haramaya University, Ethiopia. The objectives of the study was identifying the quantitative traits variation produced by induced chemical mutation; identifying the effect of mutagenic chemical on morphology, root nodules and determining the effectiveness of mutagenic chemicals in haricot bean growth. A mutagenic chemical (Hydroxylamine hydrochloride) were used as chemical mutagen. A variety of haricot bean (Haramaya G- 843) were used for inducing mutation and planted in Complete Randomized Design (CRD) factorial with three replications. A highly significant reduction at (P<0.0001) in seed germination of haramaya variety G-843 was observed at higher concentrations of Hydroxylamine hydrochloride when compared with the control. At a lower dose of mutagenic treatments, days required for flowering and days required for maturity were decreased in 0.02% concentration of Hydroxylamine hydrochloride in Haramaya variety G-843 when compared with control. The mutagenic effectiveness was found to be the highest at lower concentration in germination percentage, plant height, days of flowering and days of maturity, pods per plants, internodes length, and root nodules in Haramaya variety G-843 by hydroxylamine hydrochloride.
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