Evaluation Of Tolerance And Sensitivity Of Selected Plant Species With Special Reference To Gasoline Exhaust Pollution
Abhinav Garg, Pallavi Saxena, Chirashree Ghosh
Index Terms: Bioindicator, Chlorophyll, Gasoline Exhaust, Nitrate Reductase, Protein, Sensitive, Tolerant, VOCs
Abstract: Emissions from motor vehicle exhausts have been shown to have deleterious effects on the physiology of plant species. Our present study focuses on evaluating the tolerance and sensitivity of selected plant species viz. Dracaena deremensis (good absorber of VOCs) and Dianthus caryophyllus (susceptible to ethylene and formaldehyde (VOCs)) at selected sites which are differentiated on the basis of high gasoline exhaust emission source (Site I) and less gasoline exhaust emission source (Site II & III). For this, Air Pollution Tolerance Index (APTI) and selected physiological parameters were taken into account i.e. total chlorophyll, ascorbic acid, pH, relative water content, total protein and Nitrate reductase (NR). The results showed that D. deremensis have high chlorophyll content, ascorbic acid content, protein content, with high NR activity as compared to D. caryophyllus at all the selected sites. As per Air Pollution Tolerance Index (APTI) D. deremensis has value of 60.60, 55.25 & 55.93 at Site I, II & III respectively which comes under tolerant range and D . caryophyllus has value of 14.82, 15.41 & 15.93 at Site I, II & III respectively which comes under sensitive range. Thus, study ends up with the conclusion that D. deremensis was found to be more tolerant than D. caryophyllus at all sites and thus D. deremensis can be used as a tool in mitigation of gasoline exhaust pollution and D. caryophyllus can be used as an bioindicator for indicating gasoline exhaust pollution.
 G.L. Wheeler and G.L. Rolfe, “The relationship between daily traffic volume and the distribution of lead in roadside soil and vegetation”, Environ. Pollut, vol.18, pp. 265-274, 1979.
 J.V. Lagerwerff and A.W. Specht, “Contamination of roadside soil and vegetation with Cd, Ni, Pb and Zn”, Environ. Sci. Technol, vol.4, pp. 583-586, 1970.
 W. Burges, D.B. Berardinis and E.L. Speizer, “Exposure to automobile exhaust III. An environmental assessment”, Arch. Environ. Healt, vol. 26, pp.325-329, 1973.
 R.L. Solomon and Y.W. Hartford, “Lead and cadmium in dust and soil in a small urban community”, Environ. Sci. Technol, vol. 10, pp.773-777, 1976.
 B. Mankovska, “The content of Pb, Cd and Cl in forest trees caused by the traffic of motor vehicles”, Bio. Plantarum, pp. 414-419, 1977.
 J.G. Farmer and T.D.B. Lyon, “Lead in Glasgow street dirt and soil”, Sci. Total Environ, vol. 8, pp. 89-91, 1977.
 W. Flueckiger, S. Braun and J.J. Opertli, “Effects of air pollution caused by traffic on germination and tube growth of pollen of Nicotiana sylvestris”, Environ. Pollut, vol. 16, pp. 73-80, 1978.
 A. Banerjee, R.K. Sarkar and S. Mukherji, “Reduction in soluble protein and chlorophyll contents in a few plants as indicators of automobile exhaust pollution”, Int. J. Environ. Studies, vol. 20, pp. 239–243, 1983.
 H. Kammerbauer, H. Selinger, A. Ziegler-Joens, R. Ro¨mmmelt, D. Knoppik and B. Hock, “Toxic components of motor vehicle emissions for spruce Picea abies”, Environ. Pollut, Vol. 48, pp. 235–242, 1987b.
 J.J. Sauter, H. Kammerbauer, L. Pambor and B. Hock, “Evidence for the accelerated micromorphological degradation of epistomatal waxes in Norway spruce by motor vehicle emissions”, Eur. J. Forest Pathol, Vol. 17, pp.444–448, 1987.
 R.K. Sarker, A Banerjee and S. Mukherji, “Acceleration of peroxidise and catalase activities in leaves of dicotyledonous plants as an indication of automobile exhaust pollution”, Environ. Pollut. Vol. 41, pp. 289–295, 1986.
 T. Keller, “Auswirkungen der Strassenrandbedingungen auf physiologische Aktivitaeten der Fichte. Forstwiss”, Centralbl, 104, 312–318, 1985.
 H. Kammerbauer, A. Ziegler-Joens, H. Selinger, D. Knoppik and B. Hock, “Exposure of Norway spruce at the highway border: effects on gas exchange and growth”, Experientia. Vol. 43, pp. 1124–1125, 1987a.
 W. Fluckiger, W. Braun and H. Fluckiger-Keller, “Effect of The Interaction Between Road Salt And Road Dust Upon Water Relations Of Young Trees”, Urban Ecology, R. Bornkamm, J.A. Lee And M.R.D. Seaward, Eds. Urban Ecology. Oxford: Blackwell Scientific Publications, pp. 331-332, 1987a.
 U. Arndt, C. Franz-Gerstein, D. Hinger, J. Schachner, J. Scherrieble, F. Flores and T. Strehl, “Untersuchungen zur Eignung tropischer Planzen als Bioindikatoren in Entwicklungsla¨ndern”, VDI-Berichte, Vol. 1, 559–575, 1991.
 A. Klumpp, G. Klumpp and M. Domingos, “Plants as bioindicators of air pollution at the Serra do mar near the industrial complex of Cubatao”, Brazil. Environ. Poll, Vol. 85, pp. 109–16, 1994.
 M.S. Can˜as, H.A. Carreras, L. Orellana and M.L. Pignata, “Correlation between environmental conditions and foliar chemical parameters in Ligustrum lucidum Ait. exposed to urban air pollutants”, J. Environ. Manag, Vol. 49, pp. 167–181, 1997.
 NASA, “Clean Air Study”, http://www.scribd.com/doc/1837156/NASA-Indoor-Plants. 2010.
 J.N. Cape, “Photochemical oxidants-what else is in the atmosphere besides ozone?”, Phyton-A. Rei. Bot. A, Vol. 37, pp. 45-57, 1997.
 J.D. Hiscox and G.F. Israelstam, “A method for the extraction of chlorophyll from leaf tissue without maceration”, Can. J. Chem, Vol. 57, pp. 1332-1334, 1979.
 M.Y. Law, S.A. Charles and B. Halliwell, “Glutathione and ascorbic acid in spinach chloroplasts: The effect of hydrogen peroxide and of paraquat”, Biochem. J. Vol. 210, pp. 899-903, 1983.
 Singh, S. and Rao, D.N, “Evaluation of Plants for their Tolerance to Air Pollution”, Proceedings on Air Pollution Control, A. K. Singh Ed., New Delhi, India, pp. 218–224, 1983.
 M.M. Bradford, “Rapid and sensitive method for the quantification of protein utilizing the principle of protein-dye binding”, Anal. Biochem. Vol. 72, pp. 248-254, (1976).
 G.R. Stewart, J.A Lee and T.O. Orebamjo, “Nitrogen Metabolism of Halophytes”, New Phytol, Vol. 72, pp. 539-546, 1973.
 P. Saxena and C. Ghosh, “Variation in concentration of ozone at selected sites in Delhi”, Int. J. Env. Sci. Vol. 1(7), pp. 1899-1911, 2011.
 V.S. Tiwari and S. Peshin, “A Prominent Maximum in Surface Ozone Concentrations during Winter Months at Pune (India)”, Mausam, Vol. 46(2), pp. 155-162, 1995.
 A.R. Wellburn, O. Majernik and F.A.M. Wellburn, “Effects of SO2 and NO2 polluted air upon ultrastructure of chloroplasts”, Environ. Pollut, Vol. 3, pp. 37–49, 1972.
 A.J. Zeevart, “Some effects of fumigating plants for short periods with NO2”, Environ. Pollut. Vol. 11, pp. 97–108, 1976.
 A.R. Wellburn, “Why are atmospheric oxides of nitrogen usually phytotoxic and not alternative fertilizers”? New Phytol, Vol. 115, pp. 395–429, 1990.
 K.J. Lendzian and G. Kerstiens, “Sorption and transport of gases and vapors in plant cuticles”, Rev. Environ. Contam. Toxicol, Vol. 121, pp. 65–128, 1991.
 D. J. Spedding and W. J. Thomas, “Effect of Sulphur dioxide on the Metabolism of Glycollic acid by Barley (Hordeum vulgare) leaves”, Aust. J. Biol. Sci. Vol. 26, 2, 1973.
 J. N. B. Bell and C. H. Mudd, “Sulphur dioxide Resistance in Plants: A Case Study of Loliumperenne”, Effects of Air Pollutants on Plants, T. A. Mansfield, Eds., Cambridge: Cambridge University Press, pp. 87-103, 1976.
 T. Keller and H. Schwager, “Air Pollution and Ascorbic acid” Eur. J. Forest Pathol, Vol. 7, pp. 338-350, 1977.
 S.D. Lee, “Evaluation of Scientific Basis For Ozone (oxidants)”, Air Pollution Control Association Pittsburg, PA, 1985.
 K. Tanaka, T. Otsubo and N. Kondo, “Participation of Hydrogen Peroxide in the Inactivation of Calvin-cycle SH Enzymes in SO2-fumigated Spinach Leaves”, Plant Cell Physiol. Vol. 28, pp. 1009-1018, 1982.
 C. S. Choudhary and D. N. Rao, “Study of Some Factors in Plants Controlling their Susceptibility to SO2 Pollution”, Proceedings of the Indian National Science Academy, India, Vol. 43, 236-241, 1977.
 D.N. Rao, “Use of plants as an indicators and monitors of SO2 pollution”, Chemical Age India, Vol. 28, 655-671, 1977.
 F. Scholz and S. Reck, “Effects of Acids on Forest Trees as Measured by Titration in Vitro, Inheritance of Buffering Capacity in Picea abies”, Water, Air, Soil Pollut, Vol. 8, pp. 41-45, 1977.
 T. V. Oleinikova, “The Effect of Drought on Protoplasm Permeability in Cells of Spring Wheat Leaves”, Byull. Vses. Inst. Rasteniev, Vol. 14, pp. 25-30, 1969.
 M. Farooq and M.U. Beg, “Effect of Aqueous Sulphur dioxide on the Membrane Permeability of Common Indian tree leaves”, New Botanist, Vol. 7, pp. 213-217, 1980.
 G. Masuch, H. G. Kicinski, A. Kettrup and K. S. Boss, “Single and Combined Affects of Continuous and Discontinuous O3 and SO2 Emission on Norway spruce Needles. I. Histological and cytological changes”, Int. J. Environ. Anal. Chem, 32, pp. 213-241, 1988.