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IJSTR >> Volume 10 - Issue 5, May 2021 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Antibacterial Dynamics Of Mucor Species Against Multi-Drug Resistant Bacterial Species

[Full Text]



Sidra Noureen, Muhammad Shahzad, Ayesha Naeem, Azka Rizvi, Zunira Mughis



Antibacterial potential, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Mucor spp.



These Emergence of antibiotic resistance in pathogens poses difficulties in treatment of bacterial infection. So discovery and development of alternative antibiotics is a need of hour. Present study was designed to screen Mucor species for antibacterial activity against multi-drug resistance bacterial pathogens. Mucor isolated from animal rations (n=30) were screened for toxin production by thin layer chromatography (TLC). Non-toxigenic isolates were confirmed by polymerase chain reaction (PCR). The cell free supernatants (CFS) of isolates (n=10) were evaluated against characterized multi-drug resistant bacteria; Staphylococcus aureus, Bacillus cereus, pseudomonas aeruginosa and Escherichia coli. Seven days old cell free supernatants of Mucor species was evaluated for antibacterial activity by agar well diffusion and minimum inhibitory concentration (MIC) was determined by micro-broth dilution method. One among nine non-toxigenic Mucor species revealed antibacterial activity against MDR-bacteria with mean zone of inhibitions 8.00± 1.00, 12.00± 1.00, 7.00± 1.00 and 11.00± 1.00 for MDR Escherichia coli, S.aureus, B.cerus and P.aeruginosa respectively. The lowest MIC (6.25µL / mL) determined against MDR-S.aureus followed by 12.5 µL / mL for MDR Escherichia coli, Bcerus and P.aeruginosa. Cytotoxicity evalution on Vero cell line indicated that Mucor e ≤ 6.25µL / mL is a safe concentration. It is concluded that Mucor specie have antibacterial potential against MDR-bacterial isolates.



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