International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us

IJSTR >> Volume 4 - Issue 4, April 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Determination Of The Curcumin Pigment In Extract Curcuma Domestica Val From South Sulawesi, Indonesia, By High Performance Liquid Chromatography

[Full Text]



Sri Hastati, Veni Hadju, Gemini Alam, Nusratuddin



Index Terms: curcuma domestica val., turmeric, curcumin, hplc,



Abstract: Indonesia is a country endowed with a variety of medicinal plants with strong potential for therapeutic application, curcuma domestica val is called kunyit is one of the most popular medicinal herbs.Its important active ingredient responsible for the biological activityofcurcuma, through the major activity is antyinflammatory. Curcuma has also been reported to possess antioxidant, antiallergic,wound healing,antibacterial,antifungal and anti tumor activity.The yellow colour of curcuma is mainly due to the presence of polyphenolic curcuminoids. This experiment was aimed to determine analysis compound of curcuminoid form analysis using HPLC,LC-10AT,Shimadzu,column C-18 VP-ODS, acetonitril:acetic acid:aquabides as mobile phase,flow rate 1 ml/min and detection at 425nm.Thus the analytical method using HPLC for curcumin were feasible for quantitative analysis. The curcuminoid content from Extract Curcuma domestica Val had the highest curcumin (16.1%) followed metoxycurcumin (3.2%) and bisdemetoxycurcumin 2.8%



[1] Badan POM RI Monografi Ekstrak Tumbuhan Obat Indonesia.Raflizar, 2004.

[2] Kapoor,L.D.Handbook of ayurvedic medicinal Plants,CRC press,Boca Raton Floroda.pp185.1990

[3] Vopel,G., Gaisbaver,M and Winkler,W., Phytotherapie in der Praxis,Deutscher Arzleverlag, 74, 1990.

[4] Peres.-Almaeda,Cherubino, Alves RJ., Separation and determination of physic-chemical characteristics of curcumin, demetokxycurcumin,bisdemethoxycurcumin.Food Research International.vol38.pp1039-1044.2005

[5] World Health Organization. Rhizoma curcumae longae. In WHO Monographs on SelectedMedicinal Plants; WHO: Geneva, Switzerland, 1999; Volume 1, pp. 115-124.

[6] http://www.curcuminoids.com/page 15.htm, abinasa Corporation 2000.

[7] Roughley, P.J.; Whiting, D.A. Experiments in the biosynthesis of curcumin.J.Chem Soc. PerkinTrans. 1 pp. 2379-2388. 1973

[8] Payton, F.; Sandusky, P.; Alworth, W.L. NMR study of the solution structure of curcumin. J. Nat.Prod. pp 143-146.2007

[9] Tayyem RE, Heath DD, Al-Delaimy and Rock CL. Curcumin content of turmeric and curry powders.Nutrition and cancer pp126-131,2006

[10] Pandey A and Katiyar SK. Determination and comparison of the curcuminoid pigments in turmeric genotype (Curcuma Domestica Val) by high performance Liquid Cromatography.International journal of Pharmacy and Pharmaceutical Sciences vol.2 pp 125-132.2010

[11] Dalimartha S. Atlas Tumbuhan Obat Indonesia.Vol.2. Trubus Agriwidya

[12] Basnet P and Skalko NB., Curcumin: An Anti Inflamatory Molecule from a curry Spice on the Path to Cancer Treatment.Molecules.pp4568-4592.2011

[13] I.. Ouchemoukh, H. Louaileche, and P. Schweitzer, ”Phy Chattopadhyay, K.Biswas,U Bandyopadhyay and R.K. Banerjee. Turmeric and curcumin: Biological actions and medicinal applications. Current science vol.87 pp44-53.2004

[14] I. Stankovic. Curcumin. Chemical and Technical Assessment (CTA) FAO.,2004.

[15] W. Pothitirat and W Gritsanapan. Quantitative Analysis of curcumin,Demethoxycurcumin and bisdemethoxycurcumin in the crude Curcuminoid Extract from Curcuma Longa in Thailand by TLC-Dencitometry. Mahidol University journal of Pharmaceutical Sciences. Vol 32 pp.23-30.2005.

[16] GK.Jayaprakasha, L Rao, KK Sakariah Improved HPLC methodforthedeterminationof curcumin,demetoxycurcumin and bisdemetoxycurcumin. J Agric Food Chem vol 50.pp3668-3672

[17] TonnesenHH,Arrieta AF,Lerner D.Studies on Curcumin and Curcuminoids . Characterization of the spectroscopic Properties of the Naturally Occuring Curcuminoids and Selected Derivatives Pharmazie pp.689-693. 1995.

[18] DS Sogi, S Sharma DPS Oberoi, IA Wani. Effect of extraction parameters on curcumin yield from turmeric. J Food Sci Tech Mys.vol 47.pp300-304

[19] GK Jjayaprakasha, LJ Mohan Rao,and KK Sakariah. Improved HPLC Method for the Determination of Curcumin,Demethoxycurcumin,andBisdemethoxycurcumin. J. Agric. Food Chem., 50 (13), pp 3668–36. 2005 K

[20] Inoue,C,Nomura, S.Ito,A.Nagatsu,THino and H Oka. Purification of curcumin, demetoxycurcumin and bisdemetoksicurcumin by High speed countercurrent chromatography.J Agric Food Chem.pp.9328-36.2008

[21] J Zang, S Jinnai,R Ikeda, M Wada S Hayashida and K Nakashima. A Simple HPLC-fluorescence Method for Quantitation of Curcuminoids and Its Application to Turmeric Products. Analitic Sciences, pp.385.2009]

[22] J cheng,K weijun,L Yun,W jiabo,W haito,Lqingmiao and X Xiaohe. Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids. J.Pharm Biomed Anal.pp43-9.2010