IJSTR >> Volume 9 - Issue 3, March 2020 Edition

[Full Text]

AUTHOR(S)

Divine Joy A. Mauhay, Larry V. Padilla, Fe Corazon A. Jacinto, Eileen Z. Vitug

KEYWORDS

Hibiscus, hybridization, diagnostic character, outgroup, pollen morphology, pollen grains, variation

ABSTRACT

Hybridization of both plants and animals has innumerably benefitted man. An example of which is the numerous hybrids of Hibiscus rosa-sinensis which are primarily used for aesthetic purposes because of their colourful flowers. Phenotypic variations can already be observed in various parts of H.rosa-sinensis because of hybridization; hence, it is likely that modifications are occurring on microscopic structures such as the pollen. Through time, such variations could change the frequencies of alleles in the gene pool and could possibly lead to microevolution of the species. This study focused on the determination of variations in pollen grain morphology of ten (10) selected H. rosa-sinensis hybrids from the Institute of Plant Breeding of the University of the Philippines-Los Banos, specifically in terms of pollen aperture, size, shape, length of spine and sculpturing. The pollen shape, type of aperture and sculpturing were determined qualitatively. One-way ANOVA was employed if there is significant difference among the pollen of the hybrids in terms of the said quantitative characters. Pollen shape variation was determined through Elliptic Fourier Coefficient Analysis. Results showed that all hybrids have pantoporate type of aperture, echinate type of sculpturing, and spheroidal shape. Among the characters observed, variation was noted in their pollen size and spine length. Pollen size ranges from large to very large and long to very long spine length. Majority of the hybrids observed (7 out of 10) have very large pollen size and long pollen spines. One (1) hybrid has very large pollen size and short spines while two (2) have large pollen size and short pollen spines. There was also significant difference among the samples in terms of these characters based on statistical analysis. The hybrids with variations (Claire Baltazar x Cely Hermosa, Diamond Star and Vicky) cannot be considered outgroups on the basis of the said quantitative characters alone. Nevertheless, such variations observed should not be discounted as a possible modification in pollen morphology in progress as a result of hybridization.

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