IJSTR

International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us
CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT
QR CODE
IJSTR-QR Code

IJSTR >> Volume 3- Issue 4, April 2014 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Screening Of Diverse Organic, Inorganic And Natural Nitrogen Sources For Dextran Production By Weissella Sps Using Plackett-Burman Design

[Full Text]

 

AUTHOR(S)

B. Srinivas, P.Naga Padma

 

KEYWORDS

Keywords: Dextran, Dextransucrase, Fructose, Glucose, Plackett-Burman, Sucrose, Weissella sp

 

ABSTRACT

Abstract: Exopolysaccharides like dextran produced by different microorganisms have a wide range of applications in the food, pharmaceutical and other industries. Dextran and its derivatives like iron dextran, clinical dextran, food grade dextran are rapidly emerging as new and industrially significant products. Dextran a polymer of glucose is produced using sucrose rich media and also requires efficient nitrogen sources for production. In the present study diverse organic nitrogen sources like yeast extract, soya bean meal, meat extract, beef extract, casein hydrolysate, bacterial peptone, corn steep liquor, inorganic nitrogen sources like sodium nitrate, sodium nitrite, potassium nitrate, ammonium chloride, ammonium nitrate, ammonium sulphate, ammonium phosphate and natural nitrogen sources like Bengal gram, red gram, black gram, green gram, horse gram, soybeans, cow pea were screened using statistical design like Plackett-Burman. An eight experimental design of Plackett-Burman was used and seven sources were screened. Broth analysis indicated presence of more fructose and less glucose. Dextran was recovered from broth by alcohol precipitation. The results indicated that there was higher dextran production in organic nitrogen sources like casein hydrolysate, natural nitrogen sources like black gram. Though inorganic nitrogen sources did not show good yield, but comparatively ammonium sulphate gave a positive response. These studies indicate that organic and natural nitrogen sources can be used for optimization of production media for commercial production of dextran.

 

REFERENCES

[1]. P.Andrews,”Estimation of the molecular weight of proteins by Sephadex Gel-filteration”, Biochem.J, 91, 222-233, 1964.

[2]. J.Anthony, and M.B. Leonsins, “A Valuable plasma volume expander”, S.A. Medical Journal, 546-549, 1952.

[3]. J.Behravan, B. S. F. Bazzaz, and Z. Salimi,” Optimization of Dextran production by Leuconostoc mesenteroides NRRL B-512 using cheap and local sources of carbohydrate and nitrogen”. Biotechnology and Applied Biochemistry 38: 267-269, 2003.

[4]. M.Cortezi, R. Monti and J.Contiero, “Temperatures effects on dextransucrase production by Leuconostoc mesenteroides FT045 B isolated from alcohol and sugar mill plant”, Afr J Biotechnol, 4, 279-285, 2005.

[5]. Farwa Sarwat, Shah Ali UL Qader, Afsheen Aman and Nuzhat Ahmed, “Production and Characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713”, International Journal of Biological Sciences, 4, 379-386, 2008.

[6]. A. Lakshmi Bhavani and J. Nisha, “Dextran-The polysaccharide with versatile uses”, International Journal of Pharma and Biosciences, Vol-1, pp.569-573, 2010.

[7]. T. D. Leathers, G. T. Hayman, and G. L. Cote, “Rapid Screening of Leuconostoc mesenteroides Mutants for Elevated Proportions of Alternan to Dextran”. Current Microbiol. 31: 19-22, 1995.

[8]. N.H.Maina,M. Tenkanen, H.Maaheimo, R.Juvonen, L.Virkki, “NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weissella confuse”. Carbohydr. Res. 343:1446–1455, 2008

[9]. C.S. McClesky, L.W. Faville and Barnett, “Characteristics of Leuconostoc mesenteroides from cane sugar”, J. Bacteriol. 54, 697-708.1947.

[10]. D.L. Morris,” Quantitative determination of carbohydrates with Dreywoods Anthrone reagent”, Science, 107, 254-255, 1948.

[11]. M. Naessens, A. Cerdobbel, W. Soetaert.and E. J. Vandamme,” Leuconostoc dextransucrase and dextran;production properties and application”. J.Chem. Technol.Biotechnol.80;845-860,2005.

[12]. A.A. Onilude, O. Olaoye, I.F. Fadahunsi, A. Owoseni, E.O. Garuba and T. Atoyabi, “Effects of cultural conditions on dextran production by Leuconostoc spp”, International Food Research Journal, 20(4), 1645-1651, 2013.

[13]. R.L. Plackett and J.P.Burman, “The design of optimal multifractional experiments”. Biometrika, Vol-33.

[14]. P. J. Roe, J.N. Eistein and N.P. Goldstein, “A Photometric method for the determination of insulin in plasma and urine”, J.Biol.Chem, 178, 839, 1949.

[15]. Shah Ali UL Qader, Lubna Iqbal, Afsheen Aman, Erum Shireen and Abid Azhar, “Production of dextran by newly isolated strains of Leuconostoc mesenteroides PCSIR-4 and PCSIR-9”. Turkish Journal of Biochemistry, 31, 21-26, 2005.

[16]. A. H. Tallgren, U. Airaksinen, R. von Weissenberg, H. Ojamo, J. Kuusisto, and M. Leisola.. “Exopolysaccharide-Producing Bacteria from Sugar Beets”. Appl. Environ. Microbiol. 65, no. 2: 862-64, 1999.