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IJSTR >> Volume 4 - Issue 4, April 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Agroindustrial Byproducts For The Production Of Hyaluronic Acid By Streptococcus Zooepidemicus ATCC 39920

[Full Text]



Nicole Caldas Pan, Josiane Alessandra Vignoli, Cristiani Baldo, Hanny Cristina Braga Pereira, Rui Sérgio dos Santos Ferreira da Silva, Maria Antonia Pedrine Colabone Celligoi



Index Terms: hyaluronic acid, organic acid, soy protein, Streptococcus zooepidemicus, sugarcane molasses.



Abstract: Agroindustrial derivatives are alternative nutritional sources employed in bioprocesses that reduce costs and corroborate with social sustainability. In this study, alternative carbon (sugarcane juice, sugarcane molasses and soy molasses) and nitrogen sources (corn steep liquor, soy protein and whey protein) were evaluated for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920. The medium containing sugarcane molasses archived high yield of hyaluronic acid (0.066 g.g-1), when compared to the medium composed of glucose or sucrose. The replacement of yeast extract by soy protein was also effective for the production of the polymer resulting in 0.219 g.L-1. In general, the organic acids production was also evaluated and the results showed that the main metabolic products were lactate. In contrast, the acetate synthesis was detected only in the medium containing yeast extract. This study showed that sugarcane molasses is a promising carbon source for the hyaluronic acid production. This is the first study in which a culture media containing sugarcane molasses, a cheap substrate extensively produced in Brazil, has been successfully used for the microbial hyaluronic acid production.



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