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IJSTR >> Volume 5 - Issue 6, June 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Characterization Of Partially Purified Indigenous Lipase From Germinated Bambara Groundnut (Voan Dzeia Subterrenea).

[Full Text]

 

AUTHOR(S)

Ogueche, Nnamdi.Peter, Ikwuagwu, Emmanuel. Ogbonnaya, Onah, Benjamin. Emenike, Ugwu, Melvin Nnaemeka, Ogbu, Ambrose E.

 

KEYWORDS

Germination, characterization, lipase, purification

 

ABSTRACT

Lipase is an enzyme that catalyzes the hydrolysis of ester bonds in substrates such as phospholipids, triglycerides and cholesteryl esters. Lipase (E.C. 3.1.1.3) was extracted from the 5 days germinated bambara groundnut (Voandzeia subterrenea) using 0.1M phosphate buffer pH 7.8. The enzyme was purified using 60% ammonium sulphate saturation via precipitation followed by dialysis and column chromatography using carboxymethylcellulose. The partially purified lipase showed optimum activity at pH 10.0 and temperature of 600C. The Km and Vmax were observed to be 6mm and 1120 U/L respectively. Lipase from germinated voandzeia subterrenea has various industrial applications following its stability.

 

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