IJSTR >> Volume 7 - Issue 4, April 2018 Edition

[Full Text]

AUTHOR(S)

Febi Nadhila Nurin, Maftuch, Uun Yanuhar

KEYWORDS

Cyprinus carpio, Aeromonas hydrophila, Hermetia illucens larvae, Immunostimulant

ABSTRACT

Immunostimulant of Hermetia illucens larvae was prepared for assessment on total leukocyte, differential of leukocyte and muscle histopathology of common carp. The extract of Hermetia illucens larvae was administrated in the diet at A (4%), B (6%), C (8%), and D (10%). The extract of Hermetia illucens larvae contained of flavonoid compounds, tannins/polyphenols and alkaloids. During 30 days of the feeding trial, total leukocyte was decreased as well as decreament of differential of leukocyte at dose C (8%) also at that dose was showed the best increament of immune system of Cyprinus carpio, average of total leukocyte post-infection results was shown 1,95 x 105 sel/ml and differential of leukocyte (lymphocytes, monocytes, neutrophils) was shown 81,55%; 16,42%; 0.33%. Furthermore, the histopathological of muscle before being fed the extract showed the oedema, vacuolisation, and necrosis. The average score of the lowest muscle damage was also shown 1.27%; 1.47%; and 1.4% at dose C (8%).

REFERENCES

[1] Shirali, S., Erfani Majd, N., Mesbah, M., Seif, M.R., 2012. Histological studies of common carp ovarian development during breeding season in Khuzestan province, Iran. World J. Fish Mar. Sci. 4. 159–164.

[2] Roohi,. M. Reza, I. 2015. The Efficacy Of The Oils Of Spearmint and Methyl Salicylate as New Anesthetics and Their Effect on Glucose Levels in Common Carp (Cyprinus carpio L., 1758) Juveniles. Aquaculture 437: 327–332.

[3] Irianto, A., Hernayanti dan Budiyono. 2004. Pengaruh Suplementasi Probiotik AS-51 dalam Pakan terhadap Makrofag Ginjal Ikan Mas (Cyprinus carpio) dalam Prosiding Pengendalian Penyakit pada Ikan dan Udang Berbasis Imunisasi dan Biosecurity. Seminar Nasional Penyakit Ikan dan Udang. Purwokerto. 151–154.

[4] Wangko, S. 2014. Hermetia Illucens Aspek Forensik, Kesehatan, dan Ekonomi. Jurnal Biomedik (JBM), 6 (1): 23-29.

[5] Feng, X., Cheng, G., Chen, S.Y., Yang, H., Huang, W., 2010. Evaluation of the burn healing properties of oil extraction from housefly larva in mice. Jurnal Ethnopharmacol. 130, 586–592.

[6] Choi, W.H., Yun, J.H., Chu, J.P., Chu, K.B., 2012. Antibacterial effect of extracts of Hermetia illucens (Diptera: Stratiomyidae) larvae against Gram-negative bacteria. Entomol. Res. 42.

[7] Sugianto, D. 2007. Pengaruh Tingkat Pemberian Maggot Terhadap Pertumbuhan dan Efisiensi Pemberian Pakan Benih Ikan Gurame (Osphronemus gouramy). Thesis. Institut Pertanian Bogor. 45 pages

[8] Bijanti, R. 2005. Hematologi Ikan Teknik Pengambilan Darah dan Pemeriksaan Hematologi Ikan. Bagian Ilmu Kedokteran Dasar Veteriner. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya.

[9] Svobodova, Z., Vykusova, B. 1991. Diagnostics, prevention and therapy of fish diseases and intoxications. Research Institute of Fish Culture and Hydrobiology Vodnany, Czechoslavakia. 270 pages.

[10] Susanto, D. 2008. Gambaran Histopatologi Organ Insang, Otot, dan Usus Ikan Mas (Cyprinus carpio) di Desa Cibanteng. Thesis. Fakultas Kedokteran Hewan. Institut Pertanian Bogor. Bogor. 49 pages.

[11] Raza’I, T. S. 2008. Analisis Histopatologi Organ Insang dan Usus Ikan Kerapu Lumpur (Epinephelus coloides) yang diberi Khamir Laut (Marine Yeast) Sebagai Immunostimulan. Thesis. 95 pages.

[12] Rahman, F, A., Tetiana Haniastuti, Trianna Wahyu Utami. 2017. Skrining  okimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Vol 3 No 1. 1-7.

[13] Lestari, S.H.A., Ismoyowati, dan M. Indradji. 2013. Kajian jumlah leukosit dan diferensial leukosit pada berbagai jenis itik lokal betina yang pakannya di suplementasi probiotik. Jurnal Ilmiah Peternakan 1 (2): 699-709.

[14] Isroli, S. Susanti, E. Widiastuti, T. Yudiarti, dan Sugiharto. 2009. Observasi beberapa variable hematologis ayam kedu pada pemeliharaan intensif. Seminar Nasional Kebangkitan Peternakan, Semarang. 548-557.

[15] Napirah, A., Supadmo dan Zuprizal. 2013. Pengaruh penambahan tepung kunyit (Curcuma domestica Valet) dalam pakan terhadap parameter hematologi darah puyuh (Coturnixcoturnix japanica) pedaging. Peternakan 37 (2): 114-119.

[16] Bikrisirna, S.H.L., L.D. Mahfudz, dan N. Suthama. 2013. Ketahanan tubuh ayam broiler pada kondisi tropis yang diberi jambu biji merah (Psidium guajava) sebagai sumber antioksidan. Agromedia 31 (2):46-57.

[17] Pazra, D.F. 2008. Gambaran histopatologi insang, otot dan usus pada ikan lele (Clarias Spp.) asal dari daerah bogor. Insitut Pertanian Bogor. Thesis. 64 pages.

[18] Agung, M. 2007. Penelusuran efektifitas beberapa bahan sebagai kandidat antibakteri dalam mengatasi penyakit vibriosis pada udang windu. Fakultas Perikanan Dan Ilmu Kelautan Padjadjaran. Jatinangor. 36 pages.