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IJSTR >> Volume 9 - Issue 4, April 2020 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Development Of DNA Extraction On Mangrove Leaves

[Full Text]

 

AUTHOR(S)

Meighina Atika Istiqomah, Mohammad Basyuni, Poppy Anjelisa Zaitun Hasibuan

 

KEYWORDS

concentration, DNA, isolation, NanoVue, nucleic acids, RNA.

 

ABSTRACT

Extraction to obtain high-quality DNA is a basic principle that must be completed in molecular analysis and is one of the succesfull factors in DNA amplification to be used in genetic character analysis. This research was aimed to determine the effectiveness and purity of DNA concentration with DNA extraction of mangrove leaves of Nypa fruticans, Ceriops tagal, and Rhizophora mucronata. The parameters observed in this study are the value of DNA purity and concentration. The samples analyzed were gill and fin tissues of common carp cells. The spectrophotometric method was used to calculate DNA concentration and purity. This research was conducted in the Laboratory of Parasitology and Laboratory of Central Research, Gadjah Mada University in April 2019. Preparation samples are cell lysis, washing RNA, elucus RNA, isolation cDNA, and NanoVue operation. The purity of DNA was measured from the absorbance ratio (R) of obtained DNA from the results of this study ranging from 1.7 – 1.9. The results of DNA isolation were pure if the ratio was between 1.7 to 2.0. In this research, DNA concentration values obtained were medium (5.15 μg/ml). That good concentration (C) for PCR ranged from 0.5 to 6.5 μg/ml. High total DNA concentration values was diluted to a certain concentration to be used for the PCR amplification. Dilution of DNA concentration was carried out to the concentration of total DNA isolates to reach 5 μg/ml. Based on the purity and concentration of DNA, the DNA was used for the amplification process. The value of concentration DNA was moderate, and high value is Nypa fruticans (5.05), which is almost the same as 5-FU value as control positive (5.15). The high purity of DNA was displayed by the value of ratio absorbance to be 1.7 to 2.0, showed that the sample was not contaminated and met the requirements for further investigation and other purposes such as a starting material for technology of anti-cancer development.

 

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