International Journal of Scientific & Technology Research

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IJSTR >> Volume 9 - Issue 4, April 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Development Of Chemotherapeutic In Mangrove Leaves

[Full Text]



Taufiq Qurrohman, Poppy Anjelisa Zaitun Hasibuan, Mohammad Basyuni



concentration, DNA, isolation, NanoVue, nucleic acids, RNA.



The 2014 WHO data estimated 15,985 cases of colon cancer patients, 11,787 women in cases. The highest incidence of colorectal cancer in the country, the death rate of men in Indonesia due to colon cancer is 10.2% with 103,100 deaths, while women are 8.5% with 92,000 deaths. The use of conventional chemotherapeutic agents can cause side effects such as cardiotoxic, nausea, diarrhea, and the immune system. Therefore, it is important to look for potential anticancer alternatives including natural sources. One of the natural ingredients that has the potential to be developed as a chemotherapeutic agent is mangrove leaves. In this study, mangrove leaves from three species were extracted by maceration method using n-hexane solvent. Cytotoxic activity of n-hexane extract from three mangrove leaf species (EnHDM) was determined by linking this extract activity to WiDr cells using the MTT method EnHDM which has an IC50 value for WiDr cells. The purpose of the extract test was to obtain the smallest IC50 µg / mL, Avicennia alba (EnHDAA) which was 258.14 µg / mL and Avicennia marina (EnHDAM) that is 295.25 µg / mL Therefore, based on the flow and research method, the three extracts were used as test samples for further anticancer testing. Cytotoxic effects are shown by absorbance values, th lue for later use as a material for selectivity index testing (IS) and further testing of anticancer activity. Then in this study, the test was carried out for 24 hours then the smallest IC50 value was obtained from n-hexane extract of mangrove leaves of Avicennia lanata (EnHDAL), 243.32 ug/ml. The result showed that Avicennia lanata, Avicennia alba and Avicennia marina had cytotoxic effects on WiDr cells respectively 234.32 ug/ml, 258 ug/ml and 295,25 ug/ml this proved that it had the potential as a compound of anticancer.



[1] Diandana, R. Mengenal Seluk Beluk Kanker. Cetakan ketiga. Yogyakarta: Katahati. 15, 22, 29-30. 2009.
[2] Sudiana, K. I. Patobiologi Molekuler Kanker. Jakarta: Salemba Medika. 22–30. 2011.
[3] Kurnijasanti, R., Hamid, S.I., dan Rahmawati, K. Efek Sitotoksik In Vitro dari Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) Terhadap Kultur Sel Kanker Mieloma.Media Eksakta. 7(1): 48–54. 2008.
[4] Saenger, P., Hegerl, E.J., and Davie, J.D.S. Global Status of Mangrove Ecosystems. IUCN Commission on Ecology Papers No. 3. 88-90. 1983.
[5] Basyuni, M., Sagami, H., Baba, S., and Oku, H. Distribution, occurrenceand cluster analysis of new polyprenyl acetones and other polyisoprenoids from North Sumateran mangroves. Dendrobiology. Vol. 78: 18 – 31. 2017.
[6] Basyuni, M., Sagami, H., Baba, S., Iwasaki, H., and Oku, H. Diversity of Polyisoprenoids in Ten Okinawa Mangroves. Dendrobiology. Vol. 75: 167 – 175. 2016.
[7] Nurhanan, M.Y., Asiah. O., Ilham, MAM., Syarifah, MMS., Norhayati,I., and Sahira, HL. Antiproliferative activities of 32 Malaysian plant species in breast cancer cell lines. Jof Tropical Forest Sci.20(2): 77–81. 2008.
[8] Niranjana, R., Gayathri, R., MoSN, Sugawara, T., Hirata, T., Miyashita, K., and Ganesan, P. Carotenoids modulate the hallmarks of cancer cells. J Funct Foods. 18: 968–985. 2015.
[9] Palozza, P., Serini, S., Maggiano., N, Angelini, M., Boninsegna, A., DiNicuolo, F., Ranelletti, F.O., and Calviello G. Induction of cell cycle arrest and apoptosis inhuman colon adenocarcinoma cell lines by β-Carotene throughdown-regulation of cyclin A and Bcl-2 family proteins. Carcinogenesis .23(1): 11–18. 2002.
[10] Akter, R., Uddin S. J., Grice, I. D., and Tiralongo, E. Cytotoxic activity screening of Bangladesh imedicinal plant extracts.J Nat Med.: 1 – 7. 2009.
[11] Upadhyaya KR, Radha KS, and Madhyastha HK. Cell cycle regulation and induction of apoptosis by β-carotene in U937 and HL-60 leukemia cells. J Biochem Mol Biol. 40: 1009–1015. 2007.
[12] Gloria NF, Soares N, Brand C, Oliveira FL, Borojevic R and Teodoro AJ. Lycopene and beta-carotene induce cell-cycle arrest and apoptosis in human breast cancer cell lines. Anticancer Res. 34: 1377–1386. 2014.
[13] Sari, P. D., Basyuni, M., and Hasibuan, A. P. The Inhibiton Polyisoprenoids from Nypa fruticans leaves on cyclooxigenase 2 expression of Widr colon cancer cells. Asians Journal of Pharmaceutical and Clinical Research. 2018.
[14] Illian, N. D., Basyuni, M., and Hasibuan, A. P. Polyisoprenoids From Avicennia marina and Avicennia lanata inhibit Widr cells proliferation. Pharmacognosy Magazine. 20:1-6. 2018.
[15] Kurisaki, A., Sagami, H., and Ogura, K. Distribution of Polyprenols and Dolichols in Soybean Plant. Phytochemistry. Vol. 44: 45 – 50. 1997.
[16] Sagami, H., Kurisaki, A., Ogura, K., and Chojnacki, T. Separation of Dolichol from Dehydrodolichol by A Simple Two-Plate Thin Layer Chromatography. Journal of Lipid Research. Vol. 33: 1857–1862. 1992.
[17] Cancer Chemoprevention Research Centre, “Protokol Kultur Sel,” CCRC, http://ccrc.farmasi.ugm.ac.id/wp-content/uploads/03.007.-ujisitotoksik.pdf. 2019.
[18] Haryoto, H., Muhtadi, M., Indrayudha, P., Azizah, T., and Suhendi, A. Aktivitas Sitotoksik Ekstrak Etanol Tumbuhan Sala (Cynometraramiflora Linn) terhadap Sel HeLa, T47D and WiDr. Jurnal Penelitian Saintek. Vol. 18 (2). Surakarta: Fakultas Farmasi Universitas Muhammadiyah. 21 – 27. 2015.
[19] Ueda, J., Tezuka, Y., Banskota, A.H., Tran, Q.K., Harimaya, Y., Saiki, I., and Kadota, S. Antiproliferatif Acting of Viatnemesce Medical Plants. Biol. Pharm. Bull. 25(6): 753 – 760. 2002.
[20] Weerapreeyakul, N., Nonpunya, A., Barustux, S., Thitimetharoch, T., and Spripanidkulchai, B. Evaluation of the Anticancer Potential of Six Herbs Against a Hepatoma Cell Line. Chinese Medicine. Vol. 7 (15): 1-7. 2012.
[21] Machana, S., Weerapreeyakul, N., Barusrux, S., Nonpunya, A., Sripanidkulchai, B., and Thitimetharoch, T. Cytotoxic and Apoptotic Effects of Six Herbal Plants Against The Human Hepatocarcinoma (HepG2) Cell Line. Chinese Medicine. 6 (-): 39. 2011.
[22] Yadav, V.R., Sahdeo, P., Bokyung, S., Ramaswamy, K., and Bharat, B.A. Review: Targetting Inflammatory Pathways by Triterpenoids for Prevention and Treatment of Cancer. Toxins. 2: 2428 – 2466. 2010and W. Cristian, “Isolation and crystallization of enolase,” Biochemische Zeitschri ft, vol. 310, no. 4, pp. 384-421, 1942.