Development Of Chemotherapeutic In Mangrove Leaves

The 2014 WHO data estimated 15,985 cases of colon cancer patients, 11,787 women in cases. The highest incidence of colorectal cancer in the country, the death rate of men in Indonesia due to colon cancer is 10.2% with 103,100 deaths, while women are 8.5% with 92,000 deaths. The use of conventional chemotherapeutic agents can cause side effects such as cardiotoxic, nausea, diarrhea, and the immune system. Therefore, it is important to look for potential anticancer alternatives including natural sources. One of the natural ingredients that has the potential to be developed as a chemotherapeutic agent is mangrove leaves. In this study, mangrove leaves from three species were extracted by maceration method using n-hexane solvent. Cytotoxic activity of n-hexane extract from three mangrove leaf species (EnHDM) was determined by linking this extract activity to WiDr cells using the MTT method EnHDM which has an IC50 value for WiDr cells. The purpose of the extract test was to obtain the smallest IC50 µg / mL, Avicennia alba (EnHDAA) which was 258.14 µg / mL and Avicennia marina (EnHDAM) that is 295.25 µg / mL Therefore, based on the flow and research method, the three extracts were used as test samples for further anticancer testing. Cytotoxic effects are shown by absorbance values, th lue for later use as a material for selectivity index testing (IS) and further testing of anticancer activity. Then in this study, the test was carried out for 24 hours then the smallest IC50 value was obtained from n-hexane extract of mangrove leaves of Avicennia lanata (EnHDAL), 243.32 ug/ml. The result showed that Avicennia lanata, Avicennia alba and Avicennia marina had cytotoxic effects on WiDr cells respectively 234.32 ug/ml, 258 ug/ml and 295,25 ug/ml this proved that it had the potential as a compound of anticancer.

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