International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020


IJSTR >> Volume 9 - Issue 4, April 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Biotreatment Of Aquaculture Wastewater With Chlorella Sp. In Tubular Photo-Bioreactors

[Full Text]



Le Hung Anh, Vo Thi Kim Khuyen



microalgae, Chlorella sp., shrimp farm wastewater, tubular photo-bioreactors (PBR), Can Gio district



Microalgae are microorganisms with high performance of wastewater treatment and potential for biofuel production thanks to the ability to convert contaminants in the wastewater into their biomass. Based on these advantages, strains of Chlorella sp. isolated from the shrimp wastewater were pre-cultured in F/2 medium and later applied to treat shrimp aquaculture wastewater using tubular photo-bioreactors (PBR) in both laboratory and outdoor conditions. The wastewater collected from shrimp ponds in Can Gio coastal district was found to be suitable for the growth of Chlorella sp. (salinity 8 ‰, pH 7.33, N-NH4+ 4.32 mg/L and P-PO43- 0.12 mg/L), and a new Chlorella strain isolated from the wastewater could remove nutrients in the wastewater with high speed and efficiency. Nutrient concentrations decreased after the first 4 days of culture, wastewater treatment yields in the laboratory and outdoor culture reached 86.34% and 77.54%, respectively for ammonium removal; 72.5% and 79.1% for phosphate treatment, respectively. Furthermore, the results showed the growth of Chlorella sp. was faster in outdoor culture, compared with laboratory condition. The success of isolating Chlorella sp. strains and using them for shrimp farming wastewater treatment in tubular photo-bioreactors is the basis for further applications on the pilot scale before being implemented in practice.



[1] FAO 2014a. The State of World Fisheries and Aquaculture 2014. Rome, Italy: FAO Fisheries and Aquaculture Department.
[2] Krishna Thakur, Thitiwan Patanasatienkul, Emilie Laurin, Raphael Vanderstichel, Flavio Corsin, Larry Hammell. “Production characteristics of intensive whiteleg shrimp (Litopenaeus vannamei) farming in four Vietnam Provinces,” Aquaculture Research., vol. 49, pp. 2625–2632, 2010.
[3] Aslan, S., & Kapdan, I. K.. “Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae,” Ecological Engineering, vol. 28, no. 1, pp. 64–70, 2006. doi:http://dx.doi.org/10.1016/j.ecoleng.2006.04.003.
[4] Rawat, R. Ranjith Kumar, T. Mutanda, F. Bux. “Dual role of microalgae: Phycoremediation of domestic wastewater and biomass production for sustainable biofuels production,” Applied Energy, vol. 88, pp. 3411–3424, 2011.
[5] Olgu´ın, E.J., Galicia, S., Mercado, G., Perez, T.. “Annual productivity of Spirulina (Arthrospira) and nutrient removal in a pig wastewater recycle process under tropical conditions,” Journal of Applied Phycology, vol. 15, pp. 249–257, 2003.
[6] Dumas, A., Laliberte, G., Lessard, P., Nou¨ e, J.,. “Biotretament of fish farm effluents using the cyanobacterium Phormidium bohneri,” Aquacultural Engineering, vol. 17, pp. 57–68, 1998.
[7] Deviller, G., Aliaume, C., Nava, M.A.F., Casellas, C., Blancheton, J.P.. “High-rate algal pond treatment for water reuse in an integrated marine fish recirculating system: effect on water quality and sea bass growth,” Aquaculture, vol. 235, no. (1–4), pp. 331–344, 2004.
[8] Ivaylo Sirakov, Katya Velichkova, Stefka Stoyanova, Yordan Staykov. “The importance of microalgae for aquaculture industry. Review,” International Journal of Fisheries and Aquatic Studies, vol. 2, no. 4, pp. 81–84, 2015.
[9] Tran Chan Bac et al.. “Using wastewater from pangasius ponds to produce algal biomass of Chllorella sp. - Sử dụng nước thải ao nuôi cá tra để nuôi sinh khối tảo Chllorella sp.,” Tạp chí Khoa học Trường Đại học Cần Thơ, vol. 39, pp. 90–96, 2015.
[10] Hung, M., & Liu, J.. “Microfiltration for separation of green algae from water,” Colloids and Surfaces B: Biointerfaces, vol. 51, no. 2, pp. 157–164, 2006.
[11] Ignacio de Godos, Héctor O. Guzman, Roberto Soto, Pedro A. García-Encina, Eloy Becares, Raúl Muñoz, Virginia A. Vargas. “Coagulation/flocculation-based removal of algal–bacterial biomass from piggery wastewater treatment,” Bioresource Technology, vol. 102, pp. 923–927, 2011.
[12] Safi, C., Zebib, B., Merah, O., Pontalier, P.-Y., & Vaca-Garcia, C.. “Morphology, composition, production, processing and applications of Chlorella vulgaris: A review,” Renewable and Sustainable Energy Reviews, vol. 35, pp. 265–278, 2014.
[13] Abe, Nishmura KN, Hirano M.. “Simultaneous production of ß-carotene, vitamin E and vitamin C by the aerial microalga Trentepohia aurea,” Journal of Applied Phycology, vol. 11, pp. 33–36, 1999.