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IJSTR >> Volume 3- Issue 7, July 2014 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Effect Of Effective Microorganism (EM) On The Nutritive Quality Of Coffee Husk Silage

[Full Text]



Yonatan Kassu, Solomon Demeke, TayeTolemariam, YehenewGetachew



Index Terms: Anti-nutritional factors, chemical composition, coffee husk, EM, grass hay, IVDMD



Abstract: the experiment was conducted to study the effect of Effective Microorganisms (EM) on the nutritive quality of coffee husk silages. Eight treatment silages were produced by ensiling chopped grass hay in combination with 10, 20, 30 and 100% of dry processed coffee husk with and without the use of EM as biological inoculants. The nutritive quality of the treatments silage was evaluated on the basis of fermentation characteristics, chemical composition and in-vitro dry matter digestibility. The results obtained showed that the best silages were prepared by ensiling pure coffee husk and chopped grass hay in combination with 30% coffee husk with the use of EM as measured by visual appraisal and pH value. The pH value (4.2) obtained from the treatment silage containing 100% coffee husk with the inoculation of EM is significantly lower (P< 0.001) than that of all the others and similar to the acidity limit recommended for good quality silage. According to the results of the laboratory chemical analysis, there was significant improvement in the total ash, ether extract(EE) and crude protein(CP) content of pure coffee husk ensiled with the use of EM. The EE and CP content of the 100% coffee husk tended to decrease when it ensiled with grass hay. On the contrary, there was significant (P<0.001) decrease in cell wall (NDF, ADF and hemicelluloses) content of pure coffee husk ensiled with the use of EM as biological inoculants’. The results obtained also showed that there was significant reduction (P<0.001) in the anti-nutritional factors content (lignin, caffeine and condensed tannin) of pure coffee husk ensiled with the use of EM. Moreover there was significant improvement (P<0.001) in in-vitro dry matter digestibility of coffee husk ensiled with the EM. From the result its shows clearly that there was substantial improvement on nutritive quality of coffee husk when treated withEM. However, the output of the current result on the performance of the animal seems to be the future direction of intensive research.



[1]. Solomon D, 1991. The value of coffee pulp alone and in combination with other feeds in sheep nutrition in Ethiopia. J. Small Ruminant Research 5(3): 223-231

[2]. Alemayehu T, Esayas K and Kassu K, 2007. Coffee development and marketing improvement plan in Ethiopia. pp. 375-387. Proceeding of national workshop four decades of coffee research and development in Ethiopia, Addis Ababa, Ethiopia.

[3]. Pandey A, C. R. Soccol C R, Nigam P, Brand D, Mohan R and S. Roussos S, 2000. Biotechnological potential of coffee pulp and coffee husk for bioprocesses. Journal of Biochemical Engineering. 6:153-162

[4]. Bressani R, 1979. By-products of coffee berries. PP. 5-24. In: Brahan, J.E. and R. Bressani (eds). Coffee pulp: composition, technology and utilization. Guatemala City: Intern. Dev. Res. Center. Ottawa, Canada.

[5]. McSweeney C S Palmer B D McNeill M and Krause D.O, 2001. Microbial interaction with tannin: consequence for ruminants. Animal feed science and technology, 91:83-93

[6]. Donkoh, A., C. C. Atuahene, A. G. Kese, and B. M. Asante, 1988. The nutritional value of dried coffee pulp (DCP) in broiler chickens' diets. J.Anim. Sci. Technol. 22:139-146.

[7]. Gordon F.J, 1989. An evaluation through lactating cattle of a bacterial inoculant as an additive for grass silage. Grass and Forage Science 44:169-179.

[8]. Steen R.W J, 1989. Evaluation studies in the development of a commercial bacterial inoculant as an additive for grass silage. 3. Responses in growing cattle and interaction with protein supplementation. Grass and Forage Science 44:381-390.

[9]. HigaT and Wididana G.N. 2007. The Concept and theory of Effective Microorganism: A New Dimension for Nature Farming. pp. 20-22. In J.F. Parr, S.B. Hornick, and M.E. Simpson (eds). Proceedings of the 2nd International Conference on Kyusei Nature Farming. U.S.Department of Agriculture, Washington, D.C, USA.

[10]. EMROSA (Effective Microorganisms research organization of South Africa) 2006. Users manual. Emrosa, inc. South Africa.

[11]. Yonatan K, Solomon D and Taye T, 2011. Chemical composition and in-vitro digestibility of coffee pulp ensiled with effective microorganism in Ethiopia. Livestock Research for Rural Development. Volume 23, Article #155. Retrieved March 29, 2014, from http://www.lrrd.org/lrrd23/7/kass23155.htm

[12]. Higa T, Parr J, 1994. Beneficial and effective microorganisms for a sustainable agriculture and environment. International Nature Farming center, Atami, Japan, pp. 16

[13]. Asia-Pacific Natural Agriculture Network-APNAN, 1995. EM application manual. (First Eds.) Retrieved from http://www.agriton.nl/apnanman.html

[14]. Chantsawang S and Watcharangul P, 1999. Influence of EM on quality of poultry production. Pp.133-150. Y D A. Senanayake, and U R. Sangakkara (eds). Proceedings of the 5th International Conference on Kyusei Nature Farming, Thailand, 1998, APNAN, Thailand.

[15]. Konoplya E F and Higa T, 2000. EM application in animal husbandry-Poultry farming and its action mechanisms. Paper presented at the International Conference on EM Technology and Nature Farming, October 2000, Pyongyang DPR Korea.

[16]. Wondmeneh E., Adey M. and Tadelle D, 2011. Effect of effective microorganisms on growth parameters and serum cholesterol levels in broilers. African Journal of Agricultural Research Vol. 6(16), pp. 3841-3846. http://www.academicjournals.org/AJAR

[17]. Playne M.J and McDonald P, 1996. The Buffering constituents of herbage and of silage. J. Sci. Food Agric. 17:264-268.

[18]. AOAC, 1990. Official Method of Analysis. 15th edition. Association of Official Analytical Chemists Washington, DC. USA. 66-88p.

[19]. Goering H K and Van Soest P.J, 1970. Forage fiber analysis. Agricultural Handbook No. 379. Agricultural Research Service, USDA, Washington DC. 20 p.

[20]. Maxon, E.D., L.W. Rooney, 1972. Evaluation method for tannin analysis in sorgum grain. Ceralchem, 49:749

[21]. Abebe B, Kassahun T, Mesfin R and Araya A, 2008. Measurement of caffeine in coffee beans with UV/spectrometer. Journal of Food Chemistry, 108: 310-315

[22]. Tilley J M A and Terry R A 1963 A Two-stage technique for the in vitro digestion of forage crops. J. Brit. Grassland Soc. 18:104

[23]. SAS Institute Inc, 2000. Statistical analysis Software version 9.2, Cary, NC: SAS Institute Inc. USA.

[24]. Mannetje L. 1999. Silage Making in the Tropics with Particular Emphasis on Smallholders. Proceedings of the FAO Electronic Conference on Tropical Silage September 1-15 December 1999, Food and Agricultural Organization of the United Nations, Rome, Italy.

[25]. Bolsen K K, Ashbell G and Weinberg Z.G. 1996. Silage fermentation and silage additives. Asian-Aust. J. Anim. Sci, 92:483-493

[26]. McDonald P, Henderson A. R and Heron S.J.E, 1991. The Biochemistry of silage. 2nd(eds). halcombe Publications, Marlow, Bucks, England.340p.

[27]. Bingal, H.T and E. Baytok, 2003. The Effect of some silage additives in sorghum silage on the silage quality and ruminal degradability of nutrients. I. The effects on silage quality. Turk J. Vet.Anim.Sci, 27:15-20

[28]. Gokulakrishnan, S. Chandraraj, K. Sathyanarayana and N. Gummadi, 2005. Microbial and enzymatic methods for the removal of caffeine. Journal of Enzyme and Microbial Tech. 37:225–232.

[29]. Wong, Y.S and X. Wang, 1991. Degradation of tannins in spent coffee grounds by Pleurotussajorcaju. World J.microbiology and Biotechnology, 7:573-574

[30]. Bressani R, 1987. Anti-physiological factors in coffee pulp. PP.83-88. In: Brahan, J.E. and R. Bressani (eds). Composition, technology and utilization. Guatemala City: Institute of Nutrition of Central America and Panama.

[31]. Menezes H C F.S. Samann F S, Clifford M N and Adams M.R, 1994. The Fermentation of fresh coffee pulp for use in animal feed. Pp.52-61. Proceedings. In: COLLOQUE International sur la chimie du coffee du CACAO, 15. Montpellier, 1993, Paris: association scientific international on coffee.

[32]. Osawa R, KuroisoK,Goto S and Shimizu A, 2000. Isolation of tannin-degrading lactobacilli from humans and fermented foods. Journal of .Applied and Environmental Microbiology 66: 3093–3097

[33]. Vaquero I, Marcobal A and Munoz R 2004.Tannase activity by lactic acid bacteria isolated from grape must and wine. International Journal of Food Microbiology, 96(2):199-204

[34]. Leifa F, A. Pandey and CR. Soccol, 2000. Solid State Cultivation an Efficient Method to use Toxic Agro-Industrial Residues. Journal of Basic Microbiology, 40(3): 97-187

[35]. Clifford, M.N., J.R.Ramirezmenezes, 1991. Tannins in wet processed coffee beans and coffee pulp. J.Food Chemistry, 40:35-42.

[36]. Yamaoka-Yano, D.M. and P. Mazzafera, 1998. Degradation of caffeine by Pseudomonas putida isolated from soil. Allelopathy Journal, 5: 23-34

[37]. Silva, C.F., R.F. Scwan, E.S. Dias and A.E. Wheals, 2000. Microbial diversity during Maturation and Natural Processing of coffee cherries of coffee Arabica in Brazil. International Journal of Food Microbiology, 60: 251-260

[38]. Aquiahuatl M, Raimbaukt M, Roussos M and Trejo-Hernandez M, 1988. Solid State Fermentation of coffee pulp. pp. 13-26. In: M.Raimbault (eds). Solid State Fermentation and Bioconversion of Agro-industrial Raw materials, vol 2. ORSTOM, Montpellier.

[39]. Aramble M J and Tung R S, 1987. Evaluation of saccharomyces cerevisiae growth in the rumen ecosystem. PP. 29-32. In: 19th Biennial Conference on rumen Function, Chicago, Illinois.

[40]. Maurya M S, 1993. Effect of Feeding Live Yeast Culture (Saccharomyces cerevisiae) on rumen fermentation and nutrient digestibility in goats. A PhD Thesis. Indian Veterinary Research Institute, Deemed University, Izotnager. India.

[41]. McSwenney, C.S. and R.L. Mackie, 1997.Gastrointestinal detoxification anddigestive disorder in ruminant animals. PP. 583-634. In R.L.Mackie, B.A.White (eds). Gastrointestianl Microbiology. Vol. 1. Chapman and Hall, New York.