Production And Partial Characterization Of a Thermostable, Alkaline And Organic Solvent Tolerant Lipase From Trichoderma atroviride 676
Thiago Andrade Marques, Cristiani Baldo, Dionísio Borsato, Joao Batista Buzato, Maria Antonia Pedrine Colabone Celligoi
Index Terms: fermentation, lipase, optimization, organic solvents, thermo-alkaline, Trichoderma atroviride 676.
Abstract: The several industrial applications of lipases have stimulated interest in isolation of new enzymes from novel sources. In this study, the lipase production from Trichoderma atroviride 676 was optimized by statistical design methods. The response surface methodology based on 3(3-1) fractional factorial, showed that the yeast extract and MgSO4 played a significant role on lipase production, yielded an enzymatic activity of 101.75 U/ml. Using Box-Benhken design, the optimization of the temperature levels, pH and agitation rate resulted in the maximum enzyme production of 175.20 U/ml, obtained at 28 ºC, pH 6.0 and 105 rpm. The enzyme was optimally active at pH 8.0 and 35ºC, and was stable at pH 3.0-8.0 and temperature of 25ºC-75ºC. In addition, the lipase was highly stable on non-polar hydrophobic solvents as kerosene, n-dodecane and hexane. The new lipase from T. atroviride 676 could be considered a potential candidate for industrial and biotechnological applications.
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