Mycoflora Of Barley ( Hordeum Vulgare L.) At Different Locations In Hail Area- Saudi Arabia
Elham S. Dawood, Modhi K. Elshamry
Keywords: External mycoflora, Internal mycoflora, Aspergillus spp., Alternaria spp., Ulocladium spp.
Abstract: 400 grain samples collected from barley fields in Hail area at the northern part of Saudi Arabia was used for this study. Isolation and identification of seed-borne fungi were conducted according to standard tests described by the International Seed Testing Association (ISTA) using YGCA medium. A total of 265 of external mycoflora and 517 of internal mycoflora were grouped into five fungal genera namely, Aspergillus , , Alternaria , ; Penillium ; Fusarium and Ulocladium spp. were isolated. Comparsion between frequencies and relative densities of external and internal mycoflora was carried out among the species of the predominant genera. Aspergillus flavus and A. niger reaveled high Fr. and RD of external mycoflora (A. flavus Fr.60.9 - 40.5%, RD 48.3 - 40.9%and A. niger Fr. 52.7- 48.6- % and RD 38.7- 41.9% as external – internal mycoflora mycoflora respectively). All the species of Ulocladium and Alternaria were predominant as internal mycoflora .The most predominant species of Ulocladium and Alternaria were U. atrium (Fr 89% -75.5%and RD %-79- 62.5% as internal – external mycoflora respectively) and Alternaria alternate (Fr. 60% - 46.6 % and RD. 55-32.3as external–internal mycoflora respectively).
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