International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us

IJSTR >> Volume 4 - Issue 6, June 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Mercury Removal From Petroleum Based Industries Wastewater By P. Putida

[Full Text]



A. A. M. Azoddein, R. M. Yunus, N. M. Sulaiman, A. B. Bustary, F. A. Azli



Keywords: Pseudomonas putida, growth kinetic, mercury, petrochemical wastewater



ABSTRACT: Mercury pollution is one of a primary environmental issue and public health problem. The purpose of this research is to remove the mercury using pure culture Pseudomonas putida (ATCC 49128) at optimum growth parameters such as technique of culture, acclimatization time and speed of incubator speed. In this study, the optimum growth parameters ofP. putida were obtained to achieve the maximum of mercury removal. Thus, a field study were carried out at two different location based on petroleum industrial plants in Peninsular Malaysia. Processes involved in this research which is P. putida behavior in rehydrating free-dried growing method, growth parameters and optimum operating conditions. Analysis that carried out are turbidity, total dissolved solid and suspended solid where related to growth of P. putida. Efficiency of mercury removal from actual petroleum based industries plant 1 (P1) wastewater with the presence of 1000 ppb Hg increased from 84% after 4 hours to 90.5% after 96 hours. For plant 2, the sample with presence P. putida and nutrient broth had the highest of mercury removal which is 97.27%. This results show by using P. putida is efficient for mercury removal from actual petroleum.



[1] Gill, G.A., and Fitzgerald, W.F., 1984. Mercury sampling of open ocean at the picomolar level. Deep Sea Research.32 (3): 287 – 297.

[2] Wilhelm, S.M., and Bloom, N., 2000. Mercury in petroleum.Fuel Processing Technology.63: 1 – 27.

[3] Eisler, R., (2006). Mercury: Hazards to Living Organisms. CRC Press, Taylor & Francis Group, Boca Raton.

[4] Robledo-Ortiz, J.R., Ramirez-Arreola, D.E., Perez-Fonseca, A.A., Gomez, C., Gonzalez-Reynoso, O., Ramos-Quirarte, and Gonzalez-Nunez, R. 2011. Benzene, toluene, and o-xylene degradation by free and immobilized P. Putida Fi of postconsumer agave-fiber/polymer foamed composites. International Biodeterioration& Biodegradation.65 : 539-546.

[5] Lloyd, J.R., and Lovly, D.R. 2001. Microbial detoxification of metal and radionuclides.Current Opinion in Biotechnology.12: 248-253.

[6] Wagner-Dobler, I.,2003. Pilot plant for bioremediation of mercury-containing industrial wastewater.Appl. MicrobiolBiotechnol.62: 124 – 133.

[7] Horn, J.M., Brunke, M., Deckwer, W.D., and Timmis, K.N.,1994. Pseudomonas putida strains which constitutively overexpress mercury resistance for biodetoxification of organomercurial pollutants. Appl.And Env.Microbiol. 357 – 362.

[8] Bitton, G. 2005. Wastewater Microbiology.Third Edition. John Wiley & Sons, Inc., Publication. Florida.USA.

[9] Brock, Madigan, T.M. and Martinko, J.M. (2006). Biology of Microorganisms.Eleventh Edition.Pearson, Prentice Hall, Upper Saddle River, NJ.

[10] Felske, A.D.M., Fehr, W., Pauling, B.V., Castein, H,v., and Dobler, I.W., 2003. Functional profiling of mercuric reductase (mer A) genes in biofilm communities of a technical scale biocatalyzer. 2003. BMC Microbiology. 3(1): 22.

[11] Abuhamed, T., Bayraktar, E., Mehmetoglu, T., and Mehmetoglu, U., 2003(a). Kinetics model for growth of Pseudomonas putida F1 during benzene, toluene and phenol biodegradation.Process Biochemistry.39: 983 – 988.

[12] Kargi, F., and Eker, S., 2004. Toxicity and batch biodegradation kinetics of 2,4dichlorophenol by pure Pseudomonas putida culture. Enzyme and Microbial Technology.35: 424 – 428.

[13] Park, C.W., Kim, T.H., Kim, S.Y., Lee, J.W., and Kim, S.W., 2002. Biokinetic Parameter Estimation for degradation of 2,4,6- trinitrotoluene with Pseudomonas putida KP- T201. Journal of Bioscience and Bioengineering.94(1): 57 – 61.

[14] Tsai, S.Y., and Juang, R.S., 2006. Biodegradation of phenol and sodium salicylate mixtures by suspended Pseudomonas putida CCRC 14365.Journal of Hazardous Materials.B138, 125 – 132.

[15] Shuler, M.L. and Kargi, F. 2002. Bioprocess Engineering. Sec. Ed. PrenticeHall PTR, Prentice-Hall, Inc. Upper Saddle River, NJ .

[16] Daggett, P.M., and Simione F.P.,1987. Method of Culturing Freeze-Dried Microorganisms.US Patent., Patent No. 4672037.

[17] Sheikh, K.S.2006. Kinetics Of 2,4,6–Trinitrotoluene Reduction By Pseudomonas , Msc. Thesis, College of Engineering and Technology ofOhio University, USA.

[18] Standbury, P.F., Whitaker, A. and Hall, S. J. (1984). Principles of Fermentation Techynology.Oxford : Butterworth Heinemann.

[19] Bilotta, G.S., and Brazier, R.E. 2008. Understanding the influence of suspended solids on water quality biota.Water Research.42: 2849-2861.

[20] Metcalf and Eddy. 2003. Wastewater Engineering Treatment, Disposal and Reuse, McGraw-Hill Book Co,Singapore

[21] Lapinski, J. and Tunnacliffe, A. 2002. Reduction of suspended biomass in municipal wastewater using bdelloid rotifers.Water Research.37: 2027-2034.