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IJSTR >> Volume 5 - Issue 6, June 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



In Vitro Resistant - Induction Of Tamarillo (Solanum Betaceum Cav.) Applied By UV-B Radiation Against Anthracnose Disease By Determination Of Peroxidase And Polyphenoloxidase Activity

[Full Text]

 

AUTHOR(S)

Elimasni, Dwi Suryanto, Rosmayati, Luthfi A.M.Siregar

 

KEYWORDS

UV-B Rays, Tamarillo, Solanum betaceum Cav, Peroxidase, Polyphenoloxidase, antrachnose.

 

ABSTRACT

The aim of the research was to acquire resistance callus of Tamarillo (Solanum betaceum Cav.) which was applied by UV-B radiation against the antrachnose disease. The study was designed by Factorial randomized design, consisting of two factors: UV-B light power; 10, 20 and 30 watts, and exposure time 30, 60 and 90 seconds. The Colletorichum filtrate added into medium (25-125 ppm) as resistant selection. Callus gained by selection were analyzed by peroxidase and polyphenoloxidase activity changing. Analysis of both enzymes activity showed decreased of protein levels at all samples. But after selection of Colletorichum filtrate, both enzymes showed increasing of theirs protein levels. The samples after selection showed more tolerant against filtrate compared to control, the highest value obtained in the treatment of UV-B radiation of 20 watts and exposure time of 30 seconds for peroxidase and 20 watt and 60 seconds for polyphenoloxidase. The lowest protein content for both enzyme found on control treatments.

 

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