IJSTR >> Volume 6 - Issue 6, June 2017 Edition

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AUTHOR(S)

Habibu U. Abdu, Yayo A. Manu, Yusuf Y. Deeni

KEYWORDS

Anopheles coluzzii, Anopheles gambiae, Insecticides resistance, kdr, Malaria control, Nigeria, Susceptibility

ABSTRACT

This study was carried out to assess the level of susceptibility or resistance of sibling species of Anopheles gambiae complex from Auyo in Jigawa state between 2013 and 2014 to three classes of insecticides approved by World Health Organization (WHO) for vector control. A. gambiae larvae collected from ecologically contrasting breeding sites were reared to adults in the laboratory. Adults from the F1 progeny were assayed for resistance using the WHO adult insecticide susceptibility bioassay protocol. The mosquitoes were exposed to DDT, Permethrin and Bendiocarb belonging to 3 classes of insecticides approved by WHOPES for malaria vector control. The individual sibling species were identified molecularly and the molecular resistance mechanisms characterized by SINE and kdr PCR methods respectively. The An. gambiae populations tested were highly resistant to DDT and permethrin insecticides but less resistant to bendiocarb. Analysis of the genetic composition of the vector population revealed preponderance of An. coluzzii (> 77%). L1014F and L1014S kdr mutations correlated to insecticide resistance phenotype expression. This study indicates differential distribution of the resistant genotype of Anopheles malaria vector between ecologically different habitats in the area. The information could be useful in decision and planning making for vector control programs in the region.

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