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IJSTR >> Volume 7 - Issue 5, May 2018 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Performance Of Protease From Paddy Oats Seed Peel (Gnetum Gnemon) And Its Potential As Detergent Additive

[Full Text]

 

AUTHOR(S)

C. Indarto, Pomin Li

 

KEYWORDS

Paddy oats, protease enzyme, plant protease, detergent additive

 

ABSTRACT

Increasing demand of proteases with specific properties has lead biotechnologists to explore newer sources of proteases. Protease, enzyme that cleavage protein can be produced from microorganism, animal and plant. Several protease derived from plants, have been widely used in many industries. Although many proteases have been isolated from plants, there is no previous study of protease from paddy oats. Paddy oats seed is valuable agricultural commodity in some tropical countries, particularly in Indonesia and paddy oats seed peel is only agricultural waste. The industrial demand of protease enzymes, with appropriate specificity and stability to pH, temperature, metal ions, continues to stimulate the search for new enzyme sources. The aim of this research was to detect and to characterize protease in paddy oats seed peel as well as to evaluate its compatibility as detergent additive. To assess the effect of temperature and pH on protease activity, protease enzyme extract were examined at temperature 10, 20, 30, 40, 50, 60 and 70oC, and pH range from 3 to 9. To evaluate the compatability as detergent additive, used detergent available in the local market. To simulate washing conditions, the detergent was diluted in distilled water up to final concentration of 2 mg.mL-1. The solution was boiled for 10 minutes for the inactivation of their protease enzyme contents already present and cooled. Crude extract of protease was added to the detergent solution. The result showed optimum temperature of protease enzyme extract was 40-55oC; optimum pH 6.5-9.0; and blood stain on the fabric could be removed perfectly by detergent-protease enzyme solution in 95 minutes. This study showed that protease enzyme extracted from paddy oats seed peel hold potential as a new source of protease enzyme for biotechnological applications, in particular as detergent additive.

 

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