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IJSTR >> Volume 5 - Issue 5, May 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Extraction And Isolation Of Lycopene From Solanum Lycopersicum And Citrullus Lanatus For Bioplastic Colouring

[Full Text]



Vrinda Nair, Simran Lilwani



Lycopene, Carotenoid, UV-Visible spectrophotometer, HPLC, Bioplastic, Tomato (Solanum lycopersicum), watermelon (Citrullus lanatus).



Lycopene is a carotenoid pigment and phytochemical found in tomatoes, water melon and other fruits mostly red coloured. Due to its structure, lycopene gives its deep red colour owing to its strong colour and non- toxic in nature. Lycopene is non-polar in nature due to which it stains any porous material including plastic. Lycopene diffuses into plastic, making it difficult to remove with hot water or detergent. Since lycopene is insoluble in water but can be dissolved in organic solvent and oil. Extracting lycopene from natural source like tomato and watermelon which contains the highest amount of lycopene leads to a non-toxic colouring agent which can be used in bioplastic colouring. Identification of lycopene was carried out using UV-Visible spectrophotometer and HPLC. The lycopene content was found to be 5.50 and 11.1 (mg/kg fresh wt) in tomato and watermelon respectively. The peaks of lycopene in UV-Visible spectrophotometer were found at 459 nm and 468 nm for tomato and watermelon respectively. HPLC of lycopene was carried out which showed the λMax at 473 nm for tomato and 471 nm for watermelon. The extracted lycopene was added while preparing the bioplastic as the colouring agent. Hence, lycopene proves to be a potent biocolour for the bioplastic colouring.



[1] Aghel N, Ramezani Z, Amirfakhrian S, Isolation and quantification of lycopene from tomato cultivated in dezfoul, Iran, Jundishapur Journal of Natural Pharmaceutical Products ; 6(1): 9-15 (2011)

[2] Emmanouil H. Papaioannou and Anastasios J. Karabelas, Lycopene recovery from tomato peel under mild conditions assisted by enzymatic pre-treatment and non-ionic surfactants*, ACTABP, Vol. 59, No 1, 7174 (2012)

[3] Bioplastic, Grant from United States Department of Agriculture - National Institute of Food and Agriculture, Retrieved from Plastic Suppliers, Inc.-EarthFirst brand films, Columbus, OH: http://www.plasticsuppliers.com/index.php?p=filmguide, Ohio Corn Marketing Program, Delaware, OH: http://ohiocorn.org/ ,Univenture, Marysville, OH: http://www.univenture.com , Ohio BioProducts Innovation Center, Columbus, OH: http://bioproducts.osu.edu (2015)

[4] Neelu Malviya, Isolation and Quantification of Lycopene from Watermelon, Tomato and Papaya, Research Journal of Recent Sciences, ISSN 2277-2502, Vol. 3(IVC-2014), 68-70, (2014)

[5] Shantaram Nangude, Manisha Vite, A Simple and Sensitive RP-HPLC Method for Estimation of Lycopene in Pharmaceutical Solid Dosage Forms, Journal of pharmaceutical science and bioscientific research ((PSBR), Volume 3, Issue 1, (16-19), (2013)