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IJSTR >> Volume 5 - Issue 6, June 2016 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Physiochemical And Biological Study Of And Hemiscorpius Persicus Scorpion Venom (Birula, 1903)

[Full Text]

 

AUTHOR(S)

Abulfazl Akbari, Ahmad Taghavi Moghadam, Hadi Rabiee

 

KEYWORDS

Hemiscorpius persicus, Hemiscorpius lepturus, venom, antiserum, SDS-PAGE, Phospholipase.

 

ABSTRACT

In this study 500 specimen of Hemiscorpius persicus scorpions were hunted from South-eastern parts of Iran after morphological diagnosis of samples under stereomicroscope using diagnosis key. The scorpions were milked by electrical stimulation and the venoms were freeze dried. The average of 0.25 mg dry venom was obtained from each scorpion. The amount of soluble protein in the venom after removal of mucus was 96.4 2.3 % of crude venom. The toxicity(LD50) of crude venom was 135.336.25 g for each 18-20 gr Albino mice. The SDS-PAGE of the venom revealed 12 proteinic bands with molecular weight ranging from 3.5 to 160 kilo dalton. Phospholipase activity of the venom was determined by using egg yolk as a substrate, found to be 122 unit/mg. the neutralization test was carried out by using Razi polyvalent scorpion antivenom which revealed that 2 mg/ml of antiserum was able to neutralize the Hemiscorpius Persicus venom equivalent to 15 LD50/ml. the immunodiffusion test of antiserum versus H.P venom showed some precipitation lines which confirms the effectiveness of Razi polyvalent scorpion antivenom against the venom of H.P scorpion.

 

REFERENCES

[1] Akbari, Abolfazl et al. 1987. Scorpion fauna in Iran. Pajouhesh-va-sazandegi jour. No 34. 112-115.

[2] Akbari, Abolfazl et al. 1977. Scorpion distribution in Iran. Pajouhesh-va-sazandegi jour. No 51. 32-41

[3] Dehesa-Davila, M. Alagon, AC, Possani, LD. 1995. Clinical toxicology of scorpion stings in: Meier, G and White, G. (ed). Clinical toxicology of animal venoms and poisons. CRC Press, pp: 215-221.

[4] Farzanpay, R. 1988. A catalogue of scorpions occurring in Iran. Revue Arachnologique. 8(2): pp 33-44.

[5] Fet, V. Sissom, WD. Lowe, G. Branmalder, ME. 2000. A catalogue of scorpions of world. The New York Entomological Society. pp: 690.

[6] John, DB. 2005. Clinical laboratory methods. Mosby. pp 493-494.

[7] Latifi, M. Tabatabaai, M. 1991. Scorpion antivenom production in Iran. Loth World Congress on animal and microbial toxins. October 30.

[8] Monod, L. 2005. Hemiscorpionidae (Scorpiones) from Iran, with descriptions of two new species and notes on biogeography and phylogenetic relationships. Revue Suisse De Zoologie. 112(4): pp: 869-941.

[9] Ouchterlony. 1962. Diffusion-in-gel method for immunological analysis. . Loth World Congress on animal and microbial toxins. October 30.

[10] Radmanesh, M. 1998. Cutaneus manifestation of Hemiscorpius lepturus sting. Int. J. Dermat. Vol 37. pp: 500-507.

[11] Spearman and karber. 1977. A trimmed method for estimating median lethal concentration in toxicity bioassay. Environmental science & technology. Vol 11. No 7. 714-719.

[12] Taghavi Moghadam, A and Masihipour, B. 2008. Effect of Hemiscorpius lepturus envenomation on hematological and biochemical parameters of the patients referred to hospital. Biochem. Cell. Arch. Vol 9. No 1. pp: 5-7.