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IJSTR >> Volume 5 - Issue 6, June 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Physiochemical And Biological Study Of And Hemiscorpius Persicus Scorpion Venom (Birula, 1903)

[Full Text]



Abulfazl Akbari, Ahmad Taghavi Moghadam, Hadi Rabiee



Hemiscorpius persicus, Hemiscorpius lepturus, venom, antiserum, SDS-PAGE, Phospholipase.



In this study 500 specimen of Hemiscorpius persicus scorpions were hunted from South-eastern parts of Iran after morphological diagnosis of samples under stereomicroscope using diagnosis key. The scorpions were milked by electrical stimulation and the venoms were freeze dried. The average of 0.25 mg dry venom was obtained from each scorpion. The amount of soluble protein in the venom after removal of mucus was 96.4 2.3 % of crude venom. The toxicity(LD50) of crude venom was 135.336.25 g for each 18-20 gr Albino mice. The SDS-PAGE of the venom revealed 12 proteinic bands with molecular weight ranging from 3.5 to 160 kilo dalton. Phospholipase activity of the venom was determined by using egg yolk as a substrate, found to be 122 unit/mg. the neutralization test was carried out by using Razi polyvalent scorpion antivenom which revealed that 2 mg/ml of antiserum was able to neutralize the Hemiscorpius Persicus venom equivalent to 15 LD50/ml. the immunodiffusion test of antiserum versus H.P venom showed some precipitation lines which confirms the effectiveness of Razi polyvalent scorpion antivenom against the venom of H.P scorpion.



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