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Effect Of Extracellular Proteins (ECPS) Edwardsiella Tarda On The Innate Immune Response In Nile Tilapia (Oreochromis Niloticus)
[Full Text]
AUTHOR(S)
Cucun Herlina, Uun Yanuhar, Maftuch
KEYWORDS
Differential Leukocytes, Edwardsiella tarda, ECP, Phagocytosis, White Blood Cell.
ABSTRACT
E. tarda infects Tilapia (O. niloticus) that it has extracellular products (ECP) and immunogenic competent so it has the ability to more quickly activate the host's immune response. The first defense is carried out by the innate immune response for example the blood cells such as phagocytes, macrophages, lymphocytes and neutrophils. The purpose of research was to analyze the effect of ECP E. tarda on the innate immune response of Tilapia (O. niloticus). The methods used were ECP E. tarda isolation, vaccination, total white blood cells, differential leukocytes and phagocytic activity. The ECP treatment by peritoneal injection is 0.1 ml tail-1 for 9 days. Results showed total white blood cells differed significantly (P <0.05), the highest and lowest mean values were 29 x 103 cells ± 2.64 (Days-3 and 6) and 11 x 103 cells ± 2.25 (Days-9). The differential leukocytes showed differed significantly (P<0.05) that lymphocytes has >50%, monocytes between 23-32% and neutrophil ≤ 20%. The results of phagocytosis activity showed differed significantly (P<0.05) compared the control, the highest mean value 84.18% ± 1.81 on the 6th day. ECP causes cytokinesis which characterized by two dividing cells. ECP has a positive effect on phagocytic activity, total white blood cells, differential leukocytes and cytokinesis cell in Tilapia (O. niloticus).
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