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IJSTR >> Volume 3- Issue 7, July 2014 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Isolation And Characterization Of Hypervariable Region (HVR II) From Cellulose Synthase Gene In Neolamarckia Macrophylla

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Chia-Nin Chang, Wei-Seng Ho, Shek-Ling Pang



Index Terms: Neolamarckia macrophylla, reverse transcription-polymerase chain reaction (RT-PCR), cellulose synthase (CesA), hypervariable region II (HVRII)



Abstract: The present study is aimed to isolate and characterize the hypervariable (HVRII) region in cellulose synthase gene from the developing xylem tissues of a tropical timber tree species, Neolamarckia macrophylla. N. macrophylla is locally known as red kelampayan and it has been selected as one of the important reforestation tree species in Malaysia. RT-PCR was carried out by using the degenerate primers and one of the three amplified DNA bands was successfully sequenced and characterized. The sequence was named as NmCesA1HVRII and it was clustered in a distinct clade that is associated with secondary cell wall development. This study has generated a useful genomic resource for a better understanding about the HVRII region of CesA gene in N. macrophylla and its function which is important in future applications, genetic improvement of N. macrophylla. This also facilitates the future selection of trees with optimal cellulose content required for certain specific industries as well as synthesizing of artificial cellulose, hence increasing the economic development and growth in the country.



T. Richmond, “Higher plant cellulose synthases”, Genome Biology, vol. 1, no. 4, pp. 1-5, 2000.

[2] R. M. Brown, I. M. Saxena and K. Kudlicka, “Cellulose biosynthesis in higher plants”, Trends in Plant Science, vol. 1, pp. 149–156, 1996.

[3] S. Kimura, W. Laosinchai, T. Itoh, X. Cui, C. R. Linder and R. M. Brown, “Immunogold labeling of rosette terminal cellulose-synthesizing complexes in the vascular plant Vigna angularis”, Plant Cell, vol. 11, pp. 2075–2085, 1999.

[4] X. Liu, Q. Wang, P. Chen, F. Song, M. Guan, L. Jin, Y. Wang and C. Yang, “Four novel cellulose synthase (CESA) genes from Birch (Betula platyphylla Suk.) involved in primary and secondary cell wall biosynthesis”, International Journal of Molecular Sciences, vol. 13, pp. 12195-12212, 2012.

[5] C. H. Haigler and R. L. Blanton, “New hopes for old dreams: Evidence that plant cellulose synthase genes have finally been cloned”, Proceedings of the National Academy of Sciences of the United States of America, vol. 93, pp. 12082-12085, 1996.

[6] L. Betancur, B. Singh, R. A. Rapp, J. F. Wendel, M. D. Marks, A. W. Roberts and C. H. Haigler, “Phylogenetically distinct cellulose synthase genes support secondary wall thickening in Arabidopsis shoot trichomes and cotton fiber”, Journal of Integrative Plant Biology, vol. 52, no. 2, pp. 205-220, 2010.

[7] N. Holland, D. Holland, T. Helentjaris, K. S. Dhugga, B. Xoconostle-Cazares and D. P. Delmer, “A comparative analysis of the plant cellulose synthase (CesA) gene family”, Plant Physiology, vol. 123, pp. 1313–1323, 2000.

[8] X. Liang and C. P. Joshi, “Molecular cloning of ten distinct hypervariable regions from the cellulose synthase gene superfamily in aspen trees”, Tree Physiology, vol. 24, pp. 543-550, 2004.

[9] W.S. Ho, S.L. Pang, P. Lau and J. Ismail, “Sequence variation in the cellulose synthase (SpCesA1) gene from Shorea parvifolia ssp. parvifolia mother trees” Journal of Tropical Agricultural Science, vol. 34, no. 2, pp.323-329, 2011.

[10] S.Y. Tiong, W.S. Ho, S.L. Pang and J. Ismail, “Nucleotide diversity and association genetics of xyloglucan endotransglycosylase/ hydrolase (XTH) and cellulose synthase (CesA) genes in Neolamarckia cadamba” Journal of Biological Sciences, vol. 14, no. 4, pp. 267-375, 2014.

[11] J. E. Halawane, H. N. Hidayah and J. Kinho, “Prospek pengembangan Jabon Merah (Anthocephalus macrophyllus (Roxb.) Havil): Solusi kebutuhan kayu masa depan”, Manado: Balai Penelitian Kehutanan Manado, Badan Penelitian dan Pengembangan Kehutanan.

[12] K. A. Frazer, L. Elnitski, D. M. Church, I. Dubchak and R. C. Hardison, “Cross-species sequence comparisons: A review of methods and available resources”, Genome Research, vol. 13, pp. 1-12, 2003.

[13] E. V. Koonin and M. Y. Galperin, Sequence - evolution - function: Computational approaches in comparative genomics, Boston: Kluwer Academic.

[14] C. E. Vergara and N. C. Carpita, “β-D-glycan synthases and the CesA gene family”. Plant Molecular Biology, vol. 47, pp. 145–160, 2001.

[15] C. J. Nairn and T. Haselkorn, “Three loblolly pine CesA genes expressed in developing xylem are orthologous to secondary cell wall CesA genes of angiosperms”, New Phytologist, vol. 166, no. 3, pp. 907–915, 2005.

[16] J. R. Pear, Y. Kawagoe, W. E. Schreckengost, D. P. Delmer and D. M. Stalker, “Higher plants contain homologs of the bacterial CelA genes encoding the catalytic subunit of cellulose synthase”, Proceedings of the National Academy of Sciences of the United States of America, vol. 93, pp. 12637-12642, 1996.

[17] A. Samuga and C. P. Joshi, “A new cellulose synthase gene (PtrCesA2) from aspen xylem is orthologous to Arabidopsis AtCesA7 (irx3) gene associated with secondary cell wall synthesis”, Gene, vol. 296, no. 1-2, pp. 37-44, 2002.

[18] M. Ranik and A. A. Myburg, “Six new cellulose synthase genes from Eucalyptus are associated with primary and secondary cell wall biosynthesis”, Tree Physiology, vol. 26, pp. 545-556, 2006.

[19] E.T. Lau, W.S. Ho and A. Julaihi, “Molecular cloning of cellulose synthase gene, SpCesA1 from developing xylem of Shorea parvifolia spp. parvifolia”. Biotechnology, vol. 8, pp. 416-424, 2009.