IJSTR

International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us
CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT
QR CODE
IJSTR-QR Code

IJSTR >> Volume 6 - Issue 7, July 2017 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Enzymes Immobilization: An Overview Of Techniques, Support Materials And Its Applications

[Full Text]

 

AUTHOR(S)

Dr. Sikander Ali, Wajeeha Zafar, Sammia Shafiq, Mehvish Manzoor

 

KEYWORDS

enzyme immobilization, techniques, biotransformation, support binding, biosensors, applications.

 

ABSTRACT

With the increasing demands of world biotechnology industries, there is a need to enhance the productivity, reaction stability, reusability and shelf life of enzymes. So, novel techniques are required to facilitate large scale and economic formulations. Enzyme immobilization is done in order to meet all the challenges to enzyme activity. It provides an excellent base for increasing availability of enzyme to the substrate with greater turn over a considerable period of time. This can be done by entrapment, support binding, cross linking of enzyme crystals, etc. Several natural and synthetic support materials are used for the immobilization of enzymes. These increase the efficiency of an enzyme to a great extent. Nowadays, immobilized enzymes are preferred over their free counterpart due to their prolonged availability. Immobilized enzymes are widely used in pharmaceutical industries, cosmetic industries, food processing, biofuel production and many other sectors.

 

REFERENCES

[1]. Amine, A., H. Mohammadi, I. Bourais and G. Palleschi, 2006. Enzyme inhibition based biosensors for food safety and environmental monitoring. Biosens.Bioelectron, 21:1405-1423.

[2]. Antolin, G., F.V. Tinaut, Y. Briceno, V.Castano, C. Perez and A.I. Ramirez, 2002. Optimization of biodiesel by sunflower oil transesterification. Bioresour. Technol., 83: 111-114.

[3]. Cabrera-Padilla, R.Y., M.C. Lisboa, A.T. Fricks, E. Franceschi, A.S. Lima, D.P. Silva and C.M. Soares. 2011. Immobilization of Candida rugosa lipase on poly (3-hydroxybutyrate-co-hydroxyvalerate) a new eco-friendly support. J. Ind. Microbiol. Biotechnol.

[4]. Caprio, C., P, Gonzalez, J. Ruales and F. Batista-Viera, 2000. Bone bound enzymes for food industry application. Food Chem., 68: 403-409.

[5]. Chibata I, T. Tosa, T. Shibatani, 1992. Industrial production of optically active compounds by immobilized catalyst. Enzymologia., (Eds.: A. N. Collins, G. N. Sheldrake, J.Crosby), Wiley, New York, 1992, pp. 351 – 370.

[6]. Cunha, A.G., G. Ferna´ndez-Lorente, J.V. Bevilaqua, J. Destain, L.M. Paiva, D.M. Freire, R. Ferna´ndez-Lafuente and J.M. Guisa´n. 2008. Immobilization of Yarrowialipolytica lipase—a comparison of stability of physical adsorption and covalent attachment techniques.Appl. Biochem. Biotechnol., 146: 49–56.

[7]. D’Orazio, P., 2003. Biosensors in clinical chemistry.Clin.Chim.Acta, 334: 41-69.

[8]. D’Souza, S.F. 1998. Immobilized enzymes in bioprocess. Curr. Sci., 77: 69–79.

[9]. Dai, D and L. Xia. 2006. Effect of lipase immobilization on resolution of (R, S)-2-octanol in non aqueous media using modified ultrastable- Y molecular sieve as support. Appl. Biochem. Biotechnol., 134: 39–49.

[10]. Dais D and L. Xia. 2006. Effect of lipase immobilization on resolution of (R, S)-2-octanol in non aqueous media using modified ultrastable- Y molecular sieve as support. Appl. Biochem. Biotechnol., 134: 39–49.

[11]. Diaz J and K. J. Balkus. 1996. Probing mechanisms for enzymatic activity enhancement of organophosphorus hydrolase in functionalized mesoporous silica J. Mol. Catal. B: Enzymatic.,2, 115 – 126.

[12]. DiCosimo, R., J. McAuliffe, A.J. Poulose and G. Bohlmann. 2013. Industrial use of immobilized enzymes. Chem. Soc. Rev., 42: 6437-6474.

[13]. EI-Kaoutit, M., B. Bouchta, H. Zejli, N.Izaoumen and K.R. Temsamani, 2004. A simple conducting polymer based biosensor for the determination of atrazine. Anal.Lett., 37:1671-1681.

[14]. Galaev I.Y and B. Mattiasson.1999.'Smart' polymers and what they could do in biotechnology and medicine.Trends Biotechnol., 17, 335 – 340.

[15]. Galaev I.Y and B. Mattiasson.2004- (Eds.),Smart Polymers for Bioseparation and Bioprocessing, Taylor and Francis, London.

[16]. Gangadharan, D., K.M.Nampoothiri, S. Sivaramakrishnan and A. Pandey, 2009.Immobilized bacterial alpha amylase for effective hydrolysis of raw and soluble starch.Food Res.Int, 42: 436-442.

[17]. Giordano, R.C., M.P. Ribeiro and R.L. Bioordano, 2006. Kinetics of alpha lactumantibiotics sintesis by penicillin G acrylase (PGA) from viewpoint of the industrial enzymatic reactor optimization. Biotechnol. Adv., 24:27-41.

[18]. Gomes, C.R., R.H. Cunha, E.L. Almeida, Y.K. Chang and C.J. Steel. 2012. Effect of the emulsifier sodium stearoyllactylate and of the enzyme maltogenic amylase on the quality of pan bread during storage. Lwt-Food. Sci. Technol., 49: 96-101.

[19]. Hoa, X.D., A.G. Kirk and M. Tabrizian, 2007. Towards integrated and sensitive surface Plasmon resonance biosensors: A review of recent progress. Biosen.Bioelectron., 23: 151-160.

[20]. Huang, X.J., P.C. Chen, F. Huang, Y. Ou, M.R. Chen and Z.K. Xu . 2011. Immobilization of Candida rugosa lipase on electrospun cellulose nanofiber membrane. J. Mol. Catal. B-Enzym., 70: 95–100.

[21]. Iso, M., B. Chen, M. Eguchi, T. Kudo and S. Shrestha, 2001.Production of biodiesel fuel from triglycerides and alcohol using immobilized lipases.J. Mol. Catal. B: Enz, 16: 53-58.

[22]. Ivanov, A., G. Evtugyn, H.C. Budnikov, F. Ricci, D. Moscone and G. Palleschi, 2003b. Cholinesterase sensors based on screen printed electrode for detection of organophosphorus and carbamic pesticides. Anal.Bioanal. Chem., 377:624-631.

[23]. Ivanov, A.E., E.Edink, A.Kumar, I.Y. Galaev, A.F. Arendsen, A. Bruggink and B. Mattiasson, 2003a. Conjugation of pencillinacrylase with a reactive copolymer of N-isopropylacrylamide: A step toward a thermosensitive industrial biocatalyst. Bioethanol.Prog., 19: 1167-1175.

[24]. Jegannathan, K.R., L. Jun-Yee, E.S. Chan and P. Ravindra. 2010. Production of biodiesel from palm oil using liquid core lipase encapsulated in j-carrageenan. Fuel., 89: 2272–2277.

[25]. Jekel M, A. Buhr, T. Willke, K.-D.Vorlop, 1998.Immobilized cells.Chem. Eng. Technol., 1998, 21, 275 – 278.

[26]. Kallenberg A. I., F. van Rantwijk, R. A. Sheldon. 2005. Computational Design of a pH Stable Enzyme: Understanding Molecular Mechanism of Penicillin Acylase's Adaptation to Alkaline Conditions. Adv.Synth. Catal., 347: 905 – 926.

[27]. Karboune S, A. Archelas, R. Furstoss, J. Barratti. 2005. Nuclease p1 immobilized on deae cellulose Enzymatic. J.Mol. Catal. B., 32: 175 – 184.

[28]. Katchalski-Katzir E and D. M. Kraemer, 2000. Carrier-bound Immobilized Enzyme

[29]. Katchalski-Katzir E. and D. M. Kraemer. 2000. Immobilized TrienzymaticSystem wit Enhanced Stabilization for the Biotransformation of Lactose Enzymatic. J. Mol. Catal., 10: 57 – 176.

[30]. Khan, A.A., Husain.Q, 2007a.decolorization and removal of textile and non textile dyes from polluted waste water and dyeing effluents by using potato (solanumtuberosum) soluble and immoboilized polyphenol oxidase .Biores. Technol .98:1012-1019.

[31]. Khan, A.A., S. Akhtar and Q. Husain, 2005b.Adsoption of polyphenol oxidases on Celite 545 directly from ammonium sulphate fractionated proteins of brinjal (Solanummelongena). J. Sci.Ind. Res., 64:621-626.

[32]. Kirk O and M. W. Christensen, 2002.Lipases from Candida antarctica: Unique Biocatalysts from a Unique Origin Org. Proc. Res. Dev., 6,446 – 451.

[33]. Krajewska B. 2004. Application of chitin and chitosan-based materials for enzyme immobilizations.Enz.Microb.Technol,.35: 126 –139.

[34]. Kreiner M and M. C. Parker. 2004. Enzyme Immobilization: The Quest for Optimum Performance 87, 24 – 33.

[35]. Kubac D, A. Cejkova, J. Masak, V. Jirku, M. Lemaire, E. Gallienne, J. Bolte, R. Stloukal, L. Martinkova. 2006. Enzyme-Promoted Desymmetrisation of ProchiralBis(cyanomethyl) SulfoxideJ. Mol. Catal. B: Enzymatic2006, 39, 59 – 61.

[36]. Kurochkina, V.B. and P.S. Nys, 1999.Enzymatic synthesis of beta lactumantibiotics.I.Cephazolin.Antibiot.Khimioter., 44:12-16.

[37]. Leung., A M. Shankar and R.Mutharasan, 2007. A review of fibre-optic biosensors.Sens. Actuat. B Chem., 125: 688-703.

[38]. Lozinsky V.I, I. Y. Galaev, F. M. Plieva, I. N. Savina, H. Jungvid, B. Mattiasson. 2003. Polymeric cryogels as promising materials of biotechnological interest. Trends Biotechnol., 21, 445 – 451.

[39]. M. Kreiner, B. D. Moore, M.-C. Parker, Chem. Commun.2001, Protein-coated microcrystals for use in organic solvents: application to oxidoreductases.1096 – 1097.

[40]. Maladka, N.K., 1994. Enzymatic production of cephalexin.Enz.Microb. Technol., 16: 715-718.

[41]. Malhotra, D.B. and A. Chaubey,2003. Biosensors for clinical diagnostics industry.J. Sensors Actuat. B Chem., 91: 117-127.

[42]. Malhotra, D.B. and A. Chaubey,2003. Biosensors for clinical diagnostics industry.J. Sensors Actuat. B Chem., 91: 117-127.

[43]. Metrangolo-Ruiz De Temino D, W. Hartmeier, M. B. Ansorge-Schumacher.2005. Asymmetric Synthesis with Chemical and Biological Methods Enz.Microb. Technol.2005, 36, 3–9.

[44]. Oh, D.K., 2007. Tagatose: Properties, applications and biotechnological processes. Applied Microbiol.Biotechnol., 76: 1-8.

[45]. Petkar M., A. Lali, P. Caimi, M. Daminati. 2006. Characterization of supports activated with divinylsulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin Enzymatic.J. Mol. Catal. B. 39: 83– 90.

[46]. Petri A, P. Marconcini, P. Salvadori. 2005. Enzyme Immobilization: The Quest for Optimum Performance J. Mol. Catal. B: Enzymatic., 32, 219 – 224.

[47]. Principles,Application and Design.J. Mol. Catal. B: Enzymatic., 10, 157 – 176.

[48]. Rao, C.S., T. Sathish, P. Ravichandrand R.S. Prakasham. 2009. Characterization of thermo-and detergent stable serine protease from isolated Bacillus circulans and evaluation of eco-friendly applications. Process.Biochem., 44: 262-268.

[49]. Roy I and M. N. Gupta.2006.Immobilization of Enzymes and Cells, 2ndedn., (Ed.: J. M. Guisan), Methods in Biotechnology, Vol. 22, Humana Press, Totowa, NJ.

[50]. Roy.I, S. Sharma, M. N. Gupta. 2004. Immobilization of Enzymes and Cells Advan. Biochem. Eng. Biotechnol., 86, 159 – 189;

[51]. Sardar, M. and M.N. Gupta. 2005. Immobilization of tomato pectinase on Con A–Seralose 4B by bioaffinity layering. Enzyme Microb. Tech., 37: 355–359.

[52]. Sardar, M., I. Roy, M.N.Gupta. 2000. Simultaneous purification and immobilization of Aspergillusnigerxylanase on the reversibly soluble polymer Eudragit (TM) L-100. Enzyme Microb Tech., 27:672–679.

[53]. Sheldon, R.A. 2011. Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs). ApplMicrobiolBiotechnol., 92:467–477.

[54]. Shi, Q.H., Y. Tian, X.Y. Dong, S. Bai, Y. Sun. 2003. Chitosan-coated silica beads as immobilized metal affinity support for protein adsorption. BiochemEng J., 16: 317–322.

[55]. Spahn, C. and S.D. Minteer. 2008. Enzyme immobilization in biotechnology. Recent Pat Eng 2:195–200.

[56]. T. Masunaga, 2000. Cosmet.Sci. Technol. Ser.,25, 385–403.

[57]. Tiwari,A.K., Kumar.A, Raheman.H. 2007. Biodiesel production fromjatropha oil with high free acid:An optimized process. Biomass Bioenergy., 31:569-575.

[58]. Tonini, D., and T. Astrup. 2012. Life-cycle assessment of a waste refinery process for enzymatic treatment of municipal solid waste. Waste.Manag., 32: 165-176.

[59]. Wang, Z.G., L.S. Wan, Z.M. Liu., X.J. Huang, Z.K. Xu. 2009. Enzyme immobilization on electrospun polymer nanofibers: an overview. J MolCatal B-Enzym., 56:189–195.

[60]. Wegman, M. A., M. H. A. Janssen, F. van Rantwijk, R. A. Sheldon. 2001. A new biocatalyst: Penicillin G acylase immobilized in sol-gel micro-particles with magnetic properties. Adv. Synth. Catal., 343: 559 – 576.

[61]. Yagiz,F., Kazan.D, Akin.A.N,2007. Biodiesel production from waste oil by using lipase immobilized on hydrotactile and zeolites. Chem ,eng. J., 134:2632-267.

[62]. Yan, X., W. Li, Y.H. Ye, Recent progress on immobilization of enzymes on molecular sieves for reactions in organic solvents. Appl. Biochem. Biotechnol., 101, 113– 129.