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IJSTR >> Volume 6 - Issue 7, July 2017 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Applicability Of A Semi-Automated Clinical Chemistry Analyzer In Determining The Antioxidant Concentrations Of Selected Plants

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Allan L. Hilario, Phylis C. Rio, Geraldine Susan C. Tengco, Danilo M. Menorca



Antioxidant Assay, Semi-automated Chemistry Analyzer, A. viridis and S. edule



Plants are rich sources of antioxidants that are protective against diseases associated to oxidative stress. There is a need for high throughput screening method that should be useful in determining the antioxidant concentration in plants. Such screening method should significantly simplify and speed up most antioxidant assays. This paper aimed at comparing the applicability of a semi-automated clinical chemistry analyzer (Pointe Scientific, MI, USA) with the traditional standard curve method and using a Vis spectrophotometer in performing the DPPH assay for antioxidant screening. Samples of crude aqueous leaf extract of kulitis, Amaranthus viridis Linn, and chayote, Sechium edule Linn, were screened for the Total Antioxidant Concentration (TAC) using the two methods. Results presented in mean ± SD (μg/dl) were compared using unpaired Student’s t-test (P<0.05). All runs were done in triplicates. The mean TAC of A. viridis was 646.0 ± 45.5 μg/dl using the clinical chemistry analyzer and 581.9 ± 19.4 μg/dl using the standard curve-spectrophotometer. On the other hand, the mean TAC of S. edule was 660.2 ± 35.9 μg/dl using the semi-automated clinical chemistry analyzer and 672.3 ± 20.9 μg/dl using the spectrophotometer. No significant differences were observed between the readings of the two methods for A. viridis (P>0.05) and S. edible (P>0.05). This implies that the clinical chemistry analyzer can be an alternative method in conducting the DPPH assay to determine the TAC in plants. This study presented the applicability of a semi-automated clinical chemistry analyzer in performing the DPPH assay. Further validation can be conducted by performing other antioxidant assays using this equipment.



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