Composition And Antimicrobial Activity Of The Essential Oil Of Heracleum Lanatum Michx. From Uttarakhand Himalaya
G. C. Kharkwal, C. Pande, G. Tewari, A. Panwar, V. Pande
Index terms: Antimicrobial activity, Apiaceae, essential oil composition, Heracleum lanatum, β-phellandrene, sabinene,
Abstract: The essential oil from the flowering aerial parts of Heracleum lanatum Michx. family Apiaceae was obtained by hydrodistillation and analysed by combination of GC (RI) and GC-MS. The chemical composition of the isolated oil is reported for the first time. Sixty five compounds were identified representing 96.95% of the total oil. The chemical composition of the isolated oil was characterised by a high proportion of monoterpene hydrocarbons (39.42%), among which β-phellandrene (14.01%), sabinene (6.75%) and (E)-β-ocimene (6.45%) were the major compounds. The sesquiterpene hydrocarbons (22.50%) represented the second major fraction of the oil followed by the oxygenated sesquiterpenes (21.42%). Furthermore, antimicrobial activity of the essential oil was evaluated using agar well diffusion method.
 M. G. Pimenov and M.V. Leonov. The Asian Umbelliferae biodiversity database (ASIUM) with particular reference to South-West Asian taxa. Turk. J. Bot., 28, 139–145, 2004.
 Z. Habibi, R. Eshaghi, M. Mohammadi and M. Yousefi, Chemical composition and antimicrobial activity of essential oil of Heracleum rechingeri Manden from Iran. Nat. Prod. Res., 24, 1013-1017, 2010.
 M. K. Kaul. Himalayan Heracleum Linn. (Hogweed)- A Review. Regional Research Laboratory, Jammu-Tawi 1-18, 1990.
 N. S. Chauhan. Medicinal and Aromatic Plants of Himanchal Pradesh 2nd Edition, Indus Publication, New Delhi. 2006.
 S., Butola, R. K. Vashistha, A. R. Malik and S. S. Samant. Assessment of inter- population variability in Heracleum candicans wall with emphasis on seed characteristics and germination behaviour. J. Med. Plants Res., 4, 1523-1534, 2010.
 V. M. Negi and N. S. Chauhan. Medicinal and aromatic plants wealth of a tribal district Kinnaur in Himanchal Himalayas. Indian Forester, 6, 838-852, 2009.
 G. Iscan, F. Demirci, M. Kurkcuoglu, M. Kivanc, K. H. C. Baser. The bioactive essential oil of Heracleum sphondylium L. subsp. ternatum (Velen.) Brummit. Z. Naturforsch., 58, 195-200, 2003.
 G. Iscan, T. Ozek, G. Ozek, A. Duran, and K. H. C. Baser. Essential oils of three species of Heracleum. Anticandidal activity. Chem. Nat. Comp., 40, 544-547, 2004.
 F. Sefidkon, M. Dabiri, and N. Mohammad. Analysis of the oil of Heracleum persicum L. (seeds and stems). J. Essent. Oil Res., 16, 296-298, 2004.
 S. Guleria, R. Saini, V. Jaitak, V.K. Kaul, B. Lal, P. Rahi, A. Gulati and B. Singh, Composition and antimicrobial activity of the essential oil of Heracleum thomsonii (Clarke) from the cold desert of the western Himalayas, Nat. Prod. Res., 25, 1250-1260, 2011.
 R. P. Adams. Identification of essential oil components by gas chromatography- mass spectrometry. Carol Stream, IL; Allured Publishing Corp. 2007.
 C. Perez, M. Pauli and P. Bazerque. An antibiotic assay by the agar well diffusion method, Acta. Biolo. Med. Exper., 15, 113-115, 1990.
 C. F. Bagamboula, M. Uyttendaele, J. Debevere. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol., 21, 33-42, 2004.
 S. Karuppusamy and G. Muthuraja. Chemical composition and antioxidant activity of Heracleum sprengelianum (Wight and Arnott) essential oil growing wild in Peninsular India. Iran. J. Pharmac. Res., 10, 769-775, 2011.