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IJSTR >> Volume 5 - Issue 8, August 2016 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Molecular Based Detection of the Epstein Barr Virus and the Association with Multiple Myeloma Sudanese Patients in the Khartoum State

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Elteleb Gafer Elnaim





Background: Multiple myeloma is one of hematological malignancies, specifically lymphoproliferative disorders that characterized by normal plasma cell transformed to neoplastic plasma cell, The pad consequences follow the malignant transformation by proliferation of the neoplastic plasma cell, Previous studies describe the EBV interfering or modulating with cellular DNA repairing mechanisms and could lead to genetic changing and manipulation in the infected cells, The goal of this study was to establishing molecular based detection of the EBV in the Sudanese multiple myeloma patients by using polymerase chain reaction as one of nucleic acid amplification technology in the Khartoum-Sudan. Materials and Methods: Retrospective hospital based study was designated for molecular based detection of Epstein Barr Virus [EBV] and association with multiple myeloma Sudanese patients in the Khartoum state, in the three years from January 2014 to March 2016, in the three centers, were study 43 study population cases clinically new diagnosed for both genders with age of 40 to 75 years, the file archived specimens includes dried bone marrow aspirate and trephine block were processed to perform DAN extraction and proceeding PCR detection of BLLF1 gene. Results: population study was composed of 37 of multiple myeloma cases in group I as study group, group II with 3 lymphoma cases and group III with 3 acute myelocytic leukemia patients, these patients with confirmed diagnosis with WHO criteria of each of multiple myeloma, lymphoma and acute myelocytic leukemia consecutively. Qualitative PCR for detection of EBV genome by targeting BLLF1gene, the 37 multiple myeloma cases were show negative results P = 0.10, three cases of acute myelocytic leukemia were show negative results P = 0.21 and three cases of lymphoma were show positive result for EBV genome, BLLF1 gene P = 0.001. Conclusion: our study results were show there is no association between EBV and multiple myeloma in the two distinct bone marrow specimens. Recommendation: our study was recommended to do further study with based of RNA an expression gene analysis to give really knowledge for the association with disease transformation. Key words: Multiple myeloma, Epstein Barr virus, polymerase chain reaction, BLLF1 gene, Sudanese