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IJSTR >> Volume 8 - Issue 8, August 2019 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Screening Of Novel Antibiotic From Streptomyces Griseus To Control Pyogenic Infection Causing Multidrug Resistant Staphylococcus Aureus

[Full Text]

 

AUTHOR(S)

Saravana kumari. P, Balachandar. S, Dhanabalan. R

 

KEYWORDS

Multidrug resistance, Staphylococcus aureus, Streptomyces griseus, Antibiogram, MIC and MBC.

 

ABSTRACT

Recent studies by epidemiology surveys on acquiring the potential of drug resistance among Staphylococcus aureus delineated that drug resistance is tremendously increasing due to the high rate of mutation in virulence genes of pathogens. Apart from the emergence of Methicillin resistant Staphylococcus aureus (MRSA), Vancomycin resistant cases also increased 170% within last one decade, indicating a need for continued surveillance and to search and develop prospective agent to treat drug resistant pathogens. Present study aimed to screen for novel antibiotic from Streptomyces sp., isolated from undisturbed areas. Alkaline to acidic range of soil samples from different part of Western Ghats regions in and around Coimbatore district of Tamil Nadu were collected and upon processing thirty different Streptomyces species with unique morphological features were identified. Antibiotic production against pathogenic, multidrug resistant Staphylococcus aureus (AR, CR, ChR, CdS, ER, GR, MR, OR, VR) isolated from suppurative wound swab used in the study along with a reference strain S. aureus MTCC740 (AR, CS, ChR, CdR, ER, GS, MR, OR, VS). By the primary screening for antibiotic production from thirty Streptomyces sp., maximum zone of inhibition of 27mm showing organism belonging to Streptomyces griseus, was identified and confirmed by 16S rDNA sequencing. Antibiotic was partially purified from the S. griseus and minimal bacteriocidal concentration (MBC) of antibiotic against test pathogens S. aureus MTCC740 and clinical pathogen recorded as 128g/ml and 256g/ml respectively. Minimal inhibitory concentration (MIC) for S. aureus MTCC740 was confirmed as 8g/ml and against the clinical isolate as 32g/ml. Recorded MIC and MBC concentrations of novel antibiotics required to control the pathogens were comparatively lower than the antibiotics used in treatment at present. Thereby the study recommends the potential use of novel antibiotic purified from new strain of Streptomyces griseus as drug, after complete purification, characterization and toxicity assay as drug to treat emerging pan drug resistant clinical pathogens of S. aureus.

 

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