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IJSTR >> Volume 8 - Issue 8, August 2019 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Production, Characterization Of Proteases By Solid State Fermentation Using Sugarcane Bagasse By Warcupiella Spinulosa.

[Full Text]



Kuzhalvaymani K, Elizabeth Jacquline L , Subha T.S.



Endophytic Fungi, Protease, Solid state fermentation, Sugarcane bagasse



Solid state fermentation is well appropriate for the production of various enzymatic complexes. Microbial proteases are the most important industrial enzymes with significant applications in food, leather and pharmacy industries. In the present study the endophytic fungal strain was isolated from mangrove forest situated in Pichavaram, TamilNadu, India. The aim of this study was to confirm the ability of endophytic fungi in production of industrially important extracellular enzymes. The production of protease by Warcupiella spinulosa was studied under Solid State Fermentation, with substrates like cotton seed, sugarcane bagasse, rice straw, rice bran, wheat bran, and mixing of all the above-mentioned substrate with feathers. The results displayed that the optimum conditions for maximum protease production were found to be Sugarcane bagasse (10,616 U/ml) as the substrate, 7th day of Incubation(10,833 U/ml) , Inoculum size 3%(13,216 U/ml) and Beef extract(1%) (13,216 U/ml) as Nitrogen source, the Metal Ions Fe₂SO₄(0.1%) (14,083 U/ml) supported the high enzyme activity, at pH 7 and Temperature RT. The production of protease was carried out at various selected substrates like Sugarcanebagasse,3%, Beefextract (1%), Fe₂SO₄(0.1%) ,pH(7),Temperature(RT) ,Day-7 and the results show that best for maximum neutral protease production (20,366 U/ml). The study was proved that Warcupiella spinulosa was able to produce a very high level of protease under Solid State Fermentation using inexpensive Sugarcane bagasse.



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