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IJSTR >> Volume 8 - Issue 8, August 2019 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Preliminary Screening Of Media Formulation Through One Variable At A Time Methodology

[Full Text]

 

AUTHOR(S)

Rajesh Singh Tomar, Neha Sharma

 

KEYWORDS

One variable analysis, Medium Optimization, Response Surface Methodology, Altered Medium, Biomass.

 

ABSTRACT

Optimization of media component through changes in one variable at a time was used to enhance the biomass and antioxidant activity of selected microorganism (Escherichia coli MTCC no. 40) on different formulated media. For the present study, different types of carbon, nitrogen and mineral sources were used to formulate media composition. On the basis of absorbance, components were selected to formulate new medium and finally formulate a new medium by using all variables in one medium. It was reported that unaltered media showed 0.126 0.001 absorbance, media 1 (altered carbon source) showed 0.143 0.001, media 2 (altered nitrogen source) showed 0.150 0.001, media 3 (altered mineral source) showed 0.124 0.012 and media 4 (Altered medium) showed 0.090 0.005 absorbance at 492 nm wavelength. The component comparison and analysis was based on changes in one factor in medium. Significant result i.e. increase in biomass was reported in altered nitrogen and carbon medium compared to the unaltered medium. These components will be further used in the formulation and optimization of medium component for response surface methodology which are the primary steps involved in bioprocess technology to enhance the biomass of the particular microorganism.

 

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