Molecularauthentication Of Selected Commerciallysold Medicinal Plants In Quiapo, Manila, Philippines
Ruby Raterta, Vincent Louie D. Cabelin, Grecebio Jonathan D. Alejandro
Index Terms—DNA barcode, medicinal plants, molecular authentication,cpDNA, nrDNA
Abstract— This study aims to identify and authenticate selected medicinal plants commercially sold in Manila and assess the most effective DNA barcodes using three chloroplast markers (trnH-psbA, matK, rbcL) and a nuclear marker(ITS). Among the four evaluated markers, trnH-psbA was the most easily amplified followed by matK and ITS. The ITSmarker gave 100% sequencing success rate, followed by trnH-psbA and matK at 82% and 78%, respectively. In the BLAST analysis, matK proved to be the most successful and useful marker for identifying all samples up to the species level. For trnH-psbA, 7 out of 10 medicinal plants were identified to species level. Furthermore, the mean interspecific divergence computed using K2P revealed that matK had only 0.7% (0-1.6%),followed byITS and trnH-psbA with 0.11% (0-2.6%) and 0.16% (0-9.7%). In line with the BLAST result, matK can discriminate one species to another due to the minimal intraspecific divergence. In conclusion, matK and trnH-psbA are potential barcodes for identifying commercially sold medicinal plants where details of plant morphology are insufficient.
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