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IJSTR >> Volume 3- Issue 9, September 2014 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Molecularauthentication Of Selected Commerciallysold Medicinal Plants In Quiapo, Manila, Philippines

[Full Text]



Ruby Raterta, Vincent Louie D. Cabelin, Grecebio Jonathan D. Alejandro



Index Terms—DNA barcode, medicinal plants, molecular authentication,cpDNA, nrDNA



Abstract— This study aims to identify and authenticate selected medicinal plants commercially sold in Manila and assess the most effective DNA barcodes using three chloroplast markers (trnH-psbA, matK, rbcL) and a nuclear marker(ITS). Among the four evaluated markers, trnH-psbA was the most easily amplified followed by matK and ITS. The ITSmarker gave 100% sequencing success rate, followed by trnH-psbA and matK at 82% and 78%, respectively. In the BLAST analysis, matK proved to be the most successful and useful marker for identifying all samples up to the species level. For trnH-psbA, 7 out of 10 medicinal plants were identified to species level. Furthermore, the mean interspecific divergence computed using K2P revealed that matK had only 0.7% (0-1.6%),followed byITS and trnH-psbA with 0.11% (0-2.6%) and 0.16% (0-9.7%). In line with the BLAST result, matK can discriminate one species to another due to the minimal intraspecific divergence. In conclusion, matK and trnH-psbA are potential barcodes for identifying commercially sold medicinal plants where details of plant morphology are insufficient.



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