Evaluation Of Three Candidate DNA Barcoding Loci In Selected Ficus L. (Moraceae)
Jay Edneil C. Olivar, Rochelle Y. Brillantes, Rosario R. Rubite, Grecebio Jonathan D. Alejandro
Index Terms— DNA barcoding, ITS, Philippine Ficus, rbcL, trnH-psbA
Abstract: The genus Ficus L. (Moraceae) is known for its diverse morphology and habit as well as economical, ecological, and pharmaceutical importance. In this study, six Philippine Ficus species were utilized to determine the best barcoding loci among cpDNA (rbcL, trnH-psbA) and nrDNA (ITS) markers on the basis of universality, discriminatory power, and resolution of species. Both trnH-psbA and ITS showed 100% PCR success rate and 67% sequencing success, whereas rbcL exhibited 67% PCR success rate but 100% sequencing success. The trnH-psbA marker performed best in terms of discriminatory power, showing the highest variable informative site (71.37%) and the highest mean interspecific distance (48.32% ± 19.89%). ITS ranked next to trnH-psbA since it was able to generate 100% resolution of species and a comparable mean intraspecific distance score with trnH-psbA. Meanwhile, rbcL failed to resolve any species correctly; thus, it has a 0% resolution of species. We initially recommend trnH-psbA and ITS as potential DNA barcodes for molecular authentication of Ficus species.
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