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IJSTR >> Volume 6 - Issue 9, September 2017 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

In Vitro Efficacy Of Lactic Acid Bacteria With Antifungal Activity Against Fusarium Sp. CID124-CS Isolate From Chilli Seeds

[Full Text]



Akaram Husain, Zaiton Hassan, Asma Saleh W. El-mabrok, Mohd Nizam Lani, Mohd Bodrul Munir



Antifungal Activity, Fusarium sp. CID124-CS, Lactic Acid Bacteria (LAB), Rhizospheric soil



Lactic acid bacteria (LAB) are known as to have inhibitory activity against fungi and other pathogens. In this study LAB from soil and fermented chilli fruits were evaluated for their inhibitory activity against Fusarium sp. CID124-CS that was isolated from chilli fruits. Three LAB isolates (Lb. plantarum1-MSS, P. pentoceous1-MSS isolated from soil, one Lb. plantarum1-FCF from fermented chilli) and two from ATCC culture Lb. acidophilus ATTCC314 and Lb. plantarum ATCC8014 showed strong inhibitory activity against growth of target fungi evaluated by well diffusion assay showed high growth inhibition (6.05 mm to 7.60 mm) within 48 h at 28oC. Adding LAB supernatant to Potato Dextrose Broth (PDA) with fungi reduced mycelia growth from 36.00% to 60.00%. Similarly, fungal mass reduction with cells of LAB in De Man Rogosa and Sharpe Broth (MRSB) ranged 98.0% to 99.9% after 72 h incubation at 28oC by micro tire plate assay. Whereas, conidial germination in MEB with LAB supernatants were reduced by 93.3% to 96.6% using micro titre plate assay. This study showed that cells and CFS of LAB isolated from soil have antifungal activity and could be used as antifungal agent against Fusarium sp. CID124-CS that infect to chilli.



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