Flowering Season-Dependent Variation In The Ultrastuctural And Soluble Proteins of Achillea Wilhelmsii Mature Pollen Grains
Leila Amjad, Ahmad Majd, Mozhgan Kavianifar
Index Terms:- Achillea wilhelmsii, soluble proteins, pollen, flowering season.
Abstract:- In plant word, pollen grains have major role in sexual reprodactive cycles. In this research, we compared the ultrastructural and soluble proteins of pollen grains of Achillea wilhelmsii in early-season and late-season flowering. The pollen were collected around the city of Isfahan, Iran. The pollen ultrastructure have studied by scaning electron microscope(SEM). The pollen total proteins in both flowering season were extracted by phosphate-buffered saline and examined using Bradford assay on electrophoresis on SDS-Page. The resulte showed that the pollen grains of early-seasons flowering were oval and mature pollens in late-seasons flowering were spheroid. The pollen protein bands in early-season and late-season flowering are seem in the rang of 14.4(Kda) to 66.2(Kda), 18.4(Kda) protein band exist in early-season pollens and 66.2(Kda) protein band in late-season pollen grains.
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