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IJSTR >> Volume 1 - Issue 9, October 2012 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Production, Purification, Characterization and Comparison of Polygalacturonase from various strains of Aspergillus

[Full Text]



Niketan Deshmukh, Reshma Talkal, Kaushik Jha, Praveen Gaurav Singh, Deep Chandra Prajapati



Keywords:- Polygalacturonase (PG), Submerged fermentation, Aspergillus niger, Aspergillus flavus, Aspergillus oryzae, Ammonium sulfate



Abstract:- Polygalacturonase is an important enzyme used in food and chemical industries that processed plant material like Juice extraction, Clarification of wine, Textile etc. Fungi from genus Aspergillus are one of the most important sources of this enzyme. We compare three strains of Aspergillus (Aspergillus niger, Aspergillus flavus and Aspergillus oryzae) for production Polygalacturonase by submerged fermentation. We also characterized all the Polygalacturonase (isolated from different strains) at different parameters such as pH, Temperature, Incubation time, Concentration Substrate in medium, Concentration of enzyme etc and on the basis of these parameters it is observed that Aspergillus niger found to better strain then Aspergillus flavus and Aspergillus oryzae.



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