IJSTR

International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
0.2
2019CiteScore
 
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020

CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT

IJSTR >> Volume 2- Issue 10, October 2013 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Phytochemistry And Antibacterial Activity Of Chlorosarcinopsis Species

[Full Text]

 

AUTHOR(S)

Dhanalakshmi M, Angayarkanni, J

 

KEYWORDS

Index Terms: Antibacterial Activity, Chlorosarcinopsis sp, Phytochemical Analysis

 

ABSTRACT

Abstract: The concept of biological control for health maintenance has received widespread attention during the last few years. The present work was focused on identifying the active substances that could be used as antibacterial agents. To achieve this target, five different extracts (petroleum ether, chloroform, acetone, methanol and ethanol) of Chlorosarcinopsis sp were examined. The extracts were tested in vitro for their antibacterial effects against the bacterial sp namely Escherichia coli, Klebsiella sp, Pseudomonas sp, Salmonella, Staphylococcus aureus, Proteus sp, Methicillin-Resistant Staphylococcus aureus, Serratia sp and Bacillus sp. using paper disc diffusion method. The methanol extract were observed to inhibit the growth of all the bacteria tested. A preliminary phytochemical test revealed the presence of Alkaloids, Anthraquinones, Cardiac glycosides, Flavonoids, reducing sugars, Saponins and Terpenoids.. The present study concluded that the active metabolites present in methanol extract of the microalgae were associated with their antibacterial property.

 

REFERENCES

[1] Abdo, S.M., Hetta, M.H., Samhan, F.A., El Din RA. and Ali, G.H. Phytochemical and Antibacterial study of five freshwater algal species [J]. Asian journal of plant sciences, 2012, 11: 109-116.

[2] Ali, M.S., Saleem, M., Yamdagni, R. and Ali, M.A. Steroid and antibacterial glycosides from marine green alga Codium iyengarrii Borgesen [J]. Natural Product Letter, 2002, 16: 407-413.

[3] Bauer AW, Kirby WMM, Sherris JC, Turck M (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 36: 493-496.

[4] Beena, B.N. and Krishnika, A. Antibacterial activity of freshwater Microalgae (Scenedesmus sp.) against three bacterial strains [J]. Journal of Bioscience Research, 2011, 2(4) : 160-165.

[5] Bhagavathy, S., Sumathi, P. and Jancy Sherene Bell, I. Green algae Chlorococcum humicola-a new source of bioactive compounds with antimicrobial activity [J]. African Pacific Journal of Tropical Biomedicine, 2011, S1-S7.

[6] Bischoff, H.W. and Bold, H.C. Phycological Studies IV. Some soil algae from Enchanted Rock and related algal species [P]. Univ. Texas Publ, 1963, 6318: 1-95.

[7] Bold, H.C. The morphology of Chlamydomonas chlamydogama sp. Nov [P]. Bull. Torrey Bot. Club, 1949, 76: 101-8.

[8] Borowitzka, M.A. and Borowitzka, L.J. Vitamins and fine chemicals from micro-algae [P]. In: Borowitzka MA and Borowitzka JL (Eds) Micro-algal Biotechnology, Cambridge press, Cambridge, 1988, pp: 153-196.

[9] Cannell, R.J.P., Owsianka, A.M. and Walker, J.M. Results of a large-scale screening programme to detect antibacterial activity from freshwater algae. Br. phycol. J, 1988b, 23: 41-4.

[10] Cardozo, K.H.M., Guaratini, T., Barros, M.P., Falcão, V.R., Tonon, A.P., Lopes, N.P., Campos, S., Torres, M.A., Souza, A.O., Colepicolo, P. And Pinto, E. Metabolites from algae with economical impact. Comparative Biochemistry and Physiology. Part C, Toxicology & Pharmacology, 2007, 146:60-78.

[11] Chakraborty, K. and Lipton, A.P. Guaianesesquiterpenes from seaweed Ulva fasciata and their antibacterial properties [J]. European Journal of Medicinal Chemistry, 2010, 45: 2237-2244.

[12] Das, K., Tiwari, R.K.S. and Shrivastava, D.K. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends [J]. Journal of Medicinal Plants Research, 2010, 4: 104-111.

[13] De lara Isassi, G., Alvarez-Hernandez, S. and Collado-Vides, L. Ichtyotoxic activity of extracts from Mexico marine macroalgae [J]. J.Appl.Phycol, 2000, 12:45-52

[14] Desbois, A.P., Mearns-Spragg, A. and Smith V.J. A fatty acid from the diatom Phaeodactylum tricornutum is antibacterial against diverse bacteria including Methicilin-resistant Staphylococcus aureus (MRSA) [J]. Marine Biotechnology, 2009, 11:45-52.

[15] Ely, R.T., Supriya, C. and Naaik, G. Antimicrobial activity of marine organisms collected of the coast of South East India [J]. J. Exp. Bio. Ecol, 2004, 309:121-127

[16] Febles, C.I., Arias, A. and Gill-Rodriguez, M.C. Invitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta and Rhodophyta) collected from coast of Tenerife in Spanish [J]. Anuaria del Estudios Canarios, 1995, 34:181-192.

[17] Goud, M.L., Seshika, A.D. and Charya, M.A.S. Antibacterial activity and biomolecular composition of certain freshwater microalgae from river Godavari (India). Sci. World J, 2007, 2: 19-23.

[18] Harborne, J.B. Phytochemical methods [P]. 2nd Edn. London. Chapman and Hall, Ltd, 1973, pp: 1-7.

[19] Herrero, M., Ibañez, E., Cifuentes, A., Reglero, G. and Santoyo, S. Dunaliella salina microalga pressurized liquid extracts as potential antimicrobials [J]. Journal of Food Protection, 2006, 69:2471-2477.

[20] Justo, G.Z., Silva, M. R. and Queiroz, M.L.S. Effects of green algae chlorella vulgaris on the response of the host hematopoietic system to intraperitoneal ehrlich ascites tumour transplantation in mice [J]. Immunopharm. Immunotoxicol, 2001, 23:199-131

[21] Kaushik, P. and Chauhan, A. In vitro antibacterial activity of laboratory grown culture of Spirulina platensis [J]. Indian J. Microbiol, 2008, 48: 348-352.

[22] Khaliq-uz-zaman, S.M., Simin, K. And Shameel, M. Antimicrobial activity and phytotoxicity of sterols from Chara wallichii A. Br. (Charophyta) [J]. Pak. J. Sci. Ind. Res, 2001, 44: 301-304.

[23] Mayer, A.M.S. and Hamann, M.T. Marine pharmacology in 2001–2002: marine compounds with antihelmintic, antibacterial, anticoagulant, antidiabetic, antifungal, anti-inflammatory, antimalarial, antiplatelet, antiprotozoal, antituberculosis, and antiviral activities; affecting the cardiovascular, immune and nervous systems and other miscellaneous mechanisms of action [J]. Comparative Biochemistry and Phycology, Part C, 2005, 140:265-286.

[24] Mendes, R.L., Nobre, B.P., Cardoso, M.T., Pereira, A.P. and Palabra, A.F. Supercritical carbon dioxide extraction of compounds with pharmaceutical importance from microalgae [J]. Inorganica Chimica Acta, 2003, 356:328-334.

[25] Mendiola, J.A., Torres, C.F., Martín-Alvarez, P.J., Santoyo, S., Toré, A., Arredondo, B.O., Señoráns, F.J., Cifuentes, A. and Ibáñez, E. Use of supercritical CO2 to obtain extracts with antimicrobial activity from Chaetoceros muelleri microalga. A correlation with their lipidic content [J]. European Food Research and Technology, 2007, 224:505-510.

[26] Mtolera, M.S.P. and Semesi, A.K. Antimicrobial activity of extracts from six green algae from Tanzania [J]. Current Trends in Marine Botanical Research in the East African Region. Uppsala, Sweden, Gotab AB, 1996, pp. 211-217.

[27] Parameswaran, P.S., Bhat, K.L., Das, B.N. and Kamat, S.Y. Halogenated terpenoids from the brown alga Padina tetrastromatica (Hauck) [J]. Indian Journal of Chemistry, 1944, 33:1006-1008.

[28] Patterson, G.W. Sterols of Laminaria [J]. Comparative Biochemistry and Physiology, 1968, 24:501- 505.

[29] Pratt, R., Daniels, T.C., Eiler, J.B., Gunnison, J.B. and Kumler, W.D. Chlorellin, an antibacterial substance from Chlorella [J]. Science, 1944, 99:351-352.

[30] Rafika, C., Rym Ben, D., Hela, O., Khemissa, G. and Hatem ben, O. Antibacterial, antioxidant and cytotoxic activities of extracts from the thermophilic green alga, Chlorosarcinopsis sp [J]. African journal of Biotechnology, 2012, 11: 14844-14849.

[31] Rosa Martha Perez Gutierrez and Efren Garcia Baez. Diterpenoids from the freshwater green algae Rhizoclonium hieroglyphium with antibacterial activity [J]. Journal of Asian Natural Products and Research, 2011, 13: 934-941.

[32] Santoyo, S., Rodríguez-Meizoso, I., Cifuentes, A., Jaime, L., García-Blairsy Reina, G., Señorans, F.J. and Ibáñez, E. Green processes based on the extraction with pressurized fluids to obtain potent antimicrobials from Haematococcus pluvialis microalgae [J]. LWT – Food Science and Technology, 2009, 42:1213-1218.

[33] Sastry, V.M.V.S. and Rao, G.R.K. Antibacterial substances from marine algae: Successive extraction using Benzene, chloroform and methanol. Botanica Marina, 1994, 37: 357-360.

[34] Smith, V.J., Desbois, A.P. and Dyrynda, E.A. Conventional and unconventional antimicrobials from fish, marine invertebrates and micro-algae [J]. Marine Drugs, 2010, 8:1213-1262.

[35] Sofowara, A. Screening plants for bioactive agents [P]. In: Medicinal plants and traditional medicine in Africa. (2nd edn.) Spectrum Books Ltd. Sunshine house, Ibadan; Nigeria, 1993, pp.81-93.

[36] Souichi, O., Yasuki, S., Akira, K., Osamu, A., Teruyuki, N., Takatoshi, N., Kunihiro, K. and Hideaki, M. Antibiotic effect of linolenic acid from Chlorococcum strains HS-101 and Dunaliella primolecta on methicillin resistant Staphylococcus aureus [J]. Journal of applied phycoclogy, 1995, 7: 121-127.

[37] Taskin, E., Ozturk, M., Taskin, E. And Kurt, O. Antibacterial activities of some marine algae from the Aegean Sea (Turkey) [J]. African Journal of Biotechnology, 2007, 6:2746-2751.

[38] Trease, G. E. and Evans, W. C. Trease and Evans Pharmacognosy [P]. A physician guide to herbal medicine. 13th Edition. Bailliere Tindal, London , 1989, pp. 176-180

[39] Tuney, I., Cadirci, B., Unal, D. And Sukatar, A. Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey) [J]. Turk. J. Biol, 2006, 30:171-175.

[40] Uma, R., Sivasubramanian, V. and Niranjali Devaraj, S. Preliminary phycochemical analysis and in vitro antibacterial screening of green micro algae, Desmococcus olivaceous, Chlorococcum humicola and Chlorella vulgaris [J]. Journal of algal biomass utilization, 2011, 2(3): 74-81.