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IJSTR >> Volume 2- Issue 10, October 2013 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Antibacterial Activity Of Different Plant And Callus Extracts A Comparative Study

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V.D. Jadhav, S. M. Bhanuwanshe, S. P. Patil, D.V. Chaudhari, M.B. Adke



Keywords: Pathogens, plant extract, MS-Medium, plant growth regulators, antibacterial activity.



Abstract: Current study includes antibacterial activity of different plant and callus extract such as Momordica charantia (Karela), Cucurbita pepo (Pumpkin), Capsicum annum (Chilli), Coriander sativum (Coriander), Brassica nigra (Mustard), Nigella sativa (Black Cumin), Trigonella foenum (Fenugreek) against eight pathogenic bacteria of which five are gram positive such as Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Streptococcus sp, Bacillus megaterium and three are gram negative such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa. Plant materials were extracted using ethanol. All the extracts showed zone of inhibition against these pathogenic bacteria which were tested by agar well diffusion method. The leaf extract of Momordica charantia, Nigella sativa, Barassica nigra showed good zone of inhibition as compared to other extracts against S.aureus (13mm, 17mm,11mm), E.coli (13mm,10mm,18mm), P. aeruginosa (11mm,12mm,10mm), Streptocococcus (14mm, 15mm, 10mm), B.subtilis (10mm,17mm,10mm), P. vulgaris (15mm,18mm, 10mm), B.cereus (13mm,16mm,12mm), B.megaterium (12mm,15mm,10mm). Explants from in vitro grown plants were cultured on MS-Medium with different combination of IAA and Kinetin for callus induction. Among those combination of IAA and Kinetin (0.1 x 0.0), (1.0 x 0.0), (0.4 x 0.5), (1.0 x 0.5), (1.5 x 0.5), (0.4 x 1.0), (0.8 x1.0), (0.1 x 1.5) mg/L have showed maximum growth of calli.



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