Isolation Of Lipolytic Bacteria From Waste Contaminated Soil: A Study With Regard To Process Optimization For Lipase

Abstract: Production of valuable metabolites by micro-organisms has always been one of the main areas of intense scientific research over the years. In this regard, the present study was undertaken to isolate lipase producing bacteria from waste contaminated site and subsequent optimization of culture parameters (temperature, pH, incubation time, agitation speed, substrate specificity) for extra-cellular lipase production. Effect of carbon source, nitrogen source and metals ions were further assessed to determine the maximum lipase activity. Selection of bacterial isolates was achieved by culture in Tributyrin agar (a selective media for lipase producing bacteria). Out of 18 bacterial isolates, 2 isolates (TU-L1 and TU-L2) were found to be prominent with regard to lipolytic activity. The optimal temperature, pH and incubation time conditions for TU-L1 and TU-L2 were 45�C, 8.0, 18h; and 37�C, 7.0 and 24h respectively. The present study warrants the feasibility of isolation of industrially important microbes from waste contaminated sites.

REFERENCES

[1] R. Sharma, Y. Chisti, and U. C. Banerjee, �Production, purification, characterization, and applications of lipases,� Biotechnol. Adv. Vol. 19, 2001

[2] S. Benjamin and A. Pandey, �Optimization of liquid media for lipase production by Candida rugosa,� Bioresource Technology, vol. 55, no. 2, 1996

[3] K. E. Jaeger, S. Ransac, B. W. Dijkstra, C. Colson, M. van Heuvel, and O. Misset, �Bacterial lipases,�. FEMS Microbiology Reviews, Vol. 15, 1994

[4] E. A. Snellman, E. R. Sullivan and R. R. Colwell, �Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1,� European Journal of Biochemistry, Vol. 269, 2002

[5] E. A. Falch, �Industrial enzymes � Developments in production and application,� Biotechnol Advances, Vol. 9, 1991

[6] T. Fujii, T. Tatara, M. Minagawa, �Studies on applications of lipolytic enzyme in detergency I. Effect of lipase from Candida cylindracea on removal of olive oil from cotton fabric,� Journal of the American Oil Chemists� Society, Vol. 63, 1986

[7] R. Sharma, Y. Christi and U.C. Banerjee, �Production, purification, characterization and applications of lipases,� Biotechnology Advances, Vol. 19, 2001

[8] Y. Y. Linko, M. Lamsa, X. Wu, E. Uosukainen, J. Seppala and P. Linko, �Biodegradable products by lipase biocatalysis, � Journal of Biotechnology, Vol. 66, 1998

[9] F. Hasan, A. A. Shah and A. Hameed, �Industrial applications of microbial lipases,� Enzyme and Microbial Technology, Vol. 39, 2006

[10] E. W. Seitz, �Industrial application of microbial lipases: A review, �Journal of the American Oil Chemists� Society, Vol. 51, 1974

[11] P. Bajpai, �Application of enzymes in the pulp and paper industry,� Biotechnology Progress, Vol. 15, 1999

[12] H. Noureddini, X. Gao and R.S. Philkana, �Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil,� Bioresource Technology, Vol. 96, 2005

[13] S. Higaki, and M. Morohashi, Propionibacterium acnes lipase in seborrheic dermatitis and other skin diseases and Unsei-in. Drugs under experimental and clinical research, Vol. 29, 2003

[14] J Cardenas, E Alvarez, M-S de Castro-Alvarez, J-M Sanchez-Montero, M Valmaseda, SW Elson, and J-V Sinisterra, �Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases,� Journal of Molecular Catalysis B: Enzymatic, Vol. 14, 2001

[15] T. Godfrey, and S. West, �Introduction to Industrial Enzymology,� In: Industrial Enzymology, T. Godfrey, and S. West (Eds). 2nd Edn, Stockholm Press, New York, 1996

[16] R.G. Jensen, �Detection and determination of lipase (acylglycerol hydrolase) activity from various sources,� Lipids, Vol. 18, 1983

[17] S. J. Chen, C. Y. Cheng, T. L. Chen, �Production of an alkaline lipase by Acinetobacter radioresistens,� Journal of Fermentation and Bioengineering, Vol. 86, 1998

[18] A. Illanes, �Stability of biocatalysts,� Electronic Journal of Biotechnology, Vol. 2, 1999

[19] E. M. Qamsari, R. K. Kermanshahi, and Z. M. Nejad, �Isolation and identification of a novel, lipase-producing bacterium, Pseudomonas aeruginosa KM110,� Iranian Journal of Microbiology, Vol. 3, 2011

[20] R. Sharma, S. K. Soni, R. M. Vohra, R. S. Jolly, L. K. Gupta, J. K. Gupta, �Production of extracellular alkaline lipase from a Bacillus sp. RSJ1 and its application in ester hydrolysis,� Indian Journal of Microbiology, Vol. 42, 2002

[21] Sugihara, T. Tani, and Y. Tominaga, �Purification and characterization of a novel thermostable lipase from Bacillus sp,� Journal of Biochemistry, Vol. 109, 1991

[22] Sayari, N. Agrebi, S. Jaoua, and Y. Gargouri, �Biochemical and molecular characterization of Staphylococcus simulans lipase,� Biochimie, Vol. 83, 2001