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IJSTR >> Volume 9 - Issue 3, March 2020 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Protease Activity In Flesh Leaves Of Bidens Pilosa

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Fidele Benimana, Irina Yurievna Potoroko, Dr Uday Bagale, Maniriho Olivier, Karege Felicien



Protease activity, Casein, Bidens Pilosa, pH, protein, substrate, temperature



Proteases catalyze the hydrolysis of peptide bonds of a protein; they are present in many organisms such as plants, animals and microorganisms. Proteases perform various medicinal functions in humans they participate in wound healing, blood coagulation, digestion process and also can be used in antibody therapy and protease haves many other applications like in detergents, leather industry, pharmaceutical industry and bioremediation processes. The present study aimed at determination of protease activity from traditional medicine plant Bidens pilosa and study of incubation pH effect, Bidens Pilosa were collected in Rwasave wetlands. The leaves were ground in a mortar in an appropriate buffer. The protease activity was assayed by hydrolyzing casein as substrate by incubation at two different pH (pH4 and pH 10), at 30oC. The breakdown of casein results in releasing tyrosine. The tyrosine was colororated with the Folin-Ciocalteu reagent and read in a spectrophotometer at 660 nm and the levels were calculated, thanks to standard curve. The protease activity was expressed in mmol/mg leaves/min of incubation. The present study, shows that absorbance increase when the concentration of tyrosine increase and protease activity reported in terms of mM/mg/min in 2 different pH condition and it was found Bidens Pilosa presents higher Protease activity in acid pH 4 in comparison with alkaline pH10 its protease activity were 8.2567×10-7 mM/mg/min and 5.15×10-7 mM/mg/min respectively. This means that proteases are sensitive to pH and pH affect protease activity. The conclusion of the study states that fresh leaves of Bidens Pilosa contain proteases as common biological activity used in medicinal property and proteases from Bidens Pilosa can be used in acidic medium pH 4 than in alkaline medium pH 10.



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