IJSTR

International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
0.2
2019CiteScore
 
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020

CALL FOR PAPERS
AUTHORS
DOWNLOADS
CONTACT

IJSTR >> Volume 9 - Issue 1, January 2020 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Chlorpyrifos Removal Improvement In Liquid Media By Aspergillus Fumigatus

[Full Text]

 

AUTHOR(S)

Candra Dwi Anggreini, T. Tazkiaturrizki, Astri Rinanti

 

KEYWORDS

biodegradation, an organophosphate insecticide, chlorpyrifos, Aspergillus fumigatus, removal efficiency, biodegradator

 

ABSTRACT

The purpose of this research was to remove the chlorpyrifos exposed to liquid media by the Aspergillus fumigatus fungi. Chlorpyrifos is a toxic organophosphate insecticide with a molecular formula of C9H11Cl3NO3PS, while A. fumigatus was cultivated in potato dextrose agar (PDA) media, incubated at the a temperature of 37oC, with pH of 7 in 7 days. Furthermore, the research was initiated by variating the concentration of the microorganism at 0.5%, 1.0%, and 1.5%, impacting biodegradation into the Erlenmeyer, containing the media (PDB), pH 7, and 10% chlorpyrifos, to determine the best removal efficiency. In addition, the Erlenmeyer was place at the shaker incubator, and a rotation speed of about 180 rpm, with temperature of 25oC was maintained. Sampling was conducted every day in 5 days, and also on the 3rd, 5th, 7th, and 9th day, in order to determine the contact timing needed to generate highest efficiency, using Gas-Chromatography Mass Spectrometry (GC-MS) method. Furthermore, optimal removal was up to 99.65%, with the 1.5% A. fumigatus being exposed for 9 days in the liquid media. This research, therefore, shows the microorganism to impact degradation, as well as the potential to protect waters of environmental pollution against the impact of organophosphate pesticide.

 

REFERENCES

[1] Z. Chisti, S. Hussain, K.R. Arshad, A. Khalid and M. Arshad, “Microbial degradation of chlorpyrifos in liquid media and soil,” J. Environ Manage, vol. 114, pp. 372-80, 2013. doi: https://doi.org/10.1016/j.jenvman.2012.10.032.
[2] R.A. Gilani, M. Rafique, A. Rehman, M.F. Munis, S. Rehman and H.J. Chaudhary, “Biodegradation of chlorpyrifos by bacterial genus Pseudomonas”, J. Basic Microbiol, vol. 56, no. 2, pp. 105-19, 2016. doi: https://doi.org/10.1002/jobm.201500336.
[3] M. Farhan, A.U. Khan, A. Wahid, M. Ahmad, F. Ahmad, Z.A. Butt and A. Kanwal, “Chlorpyrifos biodegradation in laboratory soil through Bioaugmentation and its kinetics,” Asian J. Chem., vol. 25, no. 17, pp. 9994, 2013. doi: http://dx.doi.org/10.14233/ajchem.2013.15905.
[4] H. Widyatmoko, “pH Accuracy as a Parameter of the Level of Pollution of Heavy Metals in The Soil ,” Indonesian Jurnal of Urban and Environmental Technology, vol. 5, no. 5, pp. 173-178, 2011. doi: http://dx.doi.org/10.25105/urbanenvirotech.v5i5.689.
[5] B.K. Singh, A. Walker and D.J. Wright, “Bioremedial potential of fenamiphos and chlorpyrifos degrading isolates: Influence of different environmental conditions,” Soil Biol Biochem, vol. 38, pp. 2682-2693, 2006. doi: https://doi.org/10.1016/j.soilbio.2006.04.019.
[6] S. Akbar and S. Sultan, “Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement,” Braz J Microbiol, vol. 47, no. 3, pp. 563-570, 2016. doi: https://doi.org/10.1016/j.bjm.2016.04.009.
[7] R.R.M. Thengodkar and S. Sivakami, “Degradation of chlorpyrifos by an alkaline phosphatase from the cyanobacterium Spirulina platensis,” Biodegradation, vol. 21, no. 4, pp. 637–644, 2010. doi: https://doi.org/10.1007/s10532-010-9331-6
[8] E. Sulaeman, “Exploration of Bacteria Degrading Chloryrifos Insecticides in Cabbage Vegetables in West Java,” Thesis, Institut Pertanian Bogor, 2016.
[9] M. Dhanya, “Advances in microbial biodegradation of chlorpyrifos,” J. Environ Res Dev, vol. 9, no. 1, pp. 232, 2014.
[10] L.C. Vidya, M. Kumar and S. Khanna, “Biotransformation of chlorpyrifos and bioremediation of contaminated soil,” Int. Biodeterior Biodegradation, vol. 62, no. 2, pp. 204-9, 2008. doi: https://doi.org/10.1016/j.ibiod.2007.12.005.
[11] M. Supreeth and N. Raju, “Biotransformation and endosulfan by bacteria and fungi,” Appl Microbiol Biotechnol, vol. 101, no. 15, pp. 5961-71, 2017. doi: https://doi.org/10.1007/s00253-017-8401-7
[12] X. Tang, Y. Yang, W. Huang, M.B. McBride, J. Guo, R. Tao and Y. Dai, “Transformation of chlorpyrifos in integrated recirculating constructed wetlands (IRCWs) as revealed by compound-specific stable isotope (CSIA) and microbial community structure analysis,” Bioresour Technol, vol. 233 (Supplement C), pp. 264-70, 2017. doi: https://doi.org/10.1016/j.biortech.2017.02.077
[13] Sharma, J. Pandit, R. Sharma and P. Shirkot, “Biodegradation of chlorpyrifos by Pseudomonas Resinovarans strain AST2 isolated from enriched cultures,” Curr World Environ, vol. 11, no. 1, pp. 267, 2016. doi: http://dx.doi.org/10.12944/CWE.11.1.33
[14] B.M. Satria, “The use of Aspergillus niger which was Irradiated by Gamma as Bioremediator of Triazofos Residue and Heavy Metal in Onion (Allium cepa. L),” Thesis, Institut Pertanian Bogor, 2016.
[15] R. Alizadeh, L. Rafati, A.A. Ebrahimi, M.H. Ehrampoush and S.S. Khavidak, “Chlorpyrifos Bioremediation in the Environment: A Review Article,” Journal of Environmental Health and Sustainable Development, vol. 3, no. 3, pp. 606-15, 2018.
[16] S. Chen, C. Liu, C. Peng, H. Liu, M. Hu and G. Zhong, “Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-Trichloro-2 pyridinol by a new fungal strain Cladosporium cladosporioides Hu-01,” Plos One , vol. 7, pp. 10, 2012. doi: https://doi.org/10.1371/journal.pone.0047205.
[17] H. Harms, D. Schlosser, and L.Y. Wick, “Untapped potential: Exploiting fungi in bioremediation of hazardous chemicals,” Nature Rev Microbiol, vol. 9, pp. 177–192, 2011. doi: https://doi.org/10.1038/nrmicro2519.
[18] R.T. Lamar, R.B. White and K.C. Ashley, “Evaluation of white-rot fungi for the remediation of creosote contaminated soil,” Remediat, vol. J12, pp. 97-106, 2002. doi: https://doi.org/10.1002/rem.10048.
[19] J.C. Rhodes, “Aspergillus fumigatus: growth and virulence,” Medical mycology, vol. 44, no. Supplement_1, pp. S77-S81, 2006. Doi: https://doi.org/10.1080/13693780600779419.
[20] C.N. Sawyer, P.L. Mc Carty, and G.F. Parkin, Chemistry for Environmental Engineering. New York: McGraw-Hill, 4th Ed, 1994.
[21] M. Cycon, M. Wojcik and Z. Piotrowska-Seget, “Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil,” Chemosphere, vol. 76, pp. 494–501, 2009. doi: https://doi.org/10.1016/j.chemosphere.2009.03.023.
[22] K. Eunike, Astri R. and R. Ratnaningsih, “Potential of indigenous bacteria to remove cyanide with variations of contact time and temperature,” MATEC Web of Conference, vol. 197, pp. 13013, 2018. doi: https://doi.org/10.1051/matecconf/201819713013.
[23] C. Valentina, H. Rositayanti and R. Astri, “Removing cyanide by mixed culture at liquid media with variation in pH and cyanide concentration,” MATEC Web Conference, vol. 197, pp. 13016, 2018. doi: https://doi.org/10.1051/matecconf/201819713016.