International Journal of Scientific & Technology Research

Home About Us Scope Editorial Board Blog/Latest News Contact Us
10th percentile
Powered by  Scopus
Scopus coverage:
Nov 2018 to May 2020


IJSTR >> Volume 8 - Issue 10, October 2019 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Impact Of Diethyleneglycol Dibenzoate On Hormonal Activities In Zebrafish (Daniorerio)

[Full Text]



S. Subhasini, K.Sasikumar, S. Benazir Hazira, S.Senthilnathan, M. Rajasekara Pandian



Diethylene glycol dibenzoate, Reproductive, Hormones, Zebrafish, Toxicity, Endocrine disrupters, PVC



Diethylene glycol dibenzoate (DGB) is recently approved by European Chemical Agency as an alternative to phthalates in the processing of plastic. The present study focused on the changes elicited DGB by on the hormonal effects of Zebrafish. The changes in the activities of the hormones after exposure to the sub lethal doses of Diethylene glycol (1,5 and 10 ppm) referred to endocrine disruption in zebrafish.The level of Reproductive hormones significantly decreased in the treated fish in comparison with control.The results of the present study indicate that Diethylene glycol dibenzoateled to reproductive toxicity in zebrafish through hormonal measurement, which provides the basis for the estimated ecological risk during dibenzoate exposure.



[1] Lee, S., Choi, E., Baek, Y., & Lee, C. (2019). Morphology-based Banknote Fitness Determination. IEEE Access, 1– 1.doi:10.1109/access.2019.2917514
[2] A. Arukwe, “Cellular and molecular responses to endocrine- modulators and the impact on fish reproduction”. Mar. Pollut. Bull. 42 (8) pp.643 - 655, 2001.
[3] A.Arukwe, L. Fo¨rlin and A.Goksøyr, “Xenobiotic and steroid biotrans- formation enzymes in Atlantic salmon (Salmosalar) liver treated with an estrogenic compound: 4-nonylphenol”, Environmental biology and Toxicology. Chemosphere. 16, pp.2576 - 2583, 1997
[4] T.Andersson, M.Pesonen and C.Johansson, “Differential induction of cytochrome P450–dependent monooxygenase, epoxidehydrolase, glutathione transferase and UDP glucuronosyltransferase activities in the liver of the rainbow trout by þ-naphthoflavone or Clophen A50”, Journal of Biochem. Pharmacol. 34, pp.3309–3314, 1985.
[5] AM. Api,“Toxicological prowled of diethylphthalate: a vehicle for fragrance and cosmetic ingredients”, Journal of Food Chemical Toxicology. 39, pp.97– 108, 2001
[6] B. Borg, “Androgens in teleost fishes”. Comp Biochem Physiol, 10, pp.219–45, 1994
[7] D.ColonI.Caro, CJ.Bourdony and O.Rosario “Identification of phthalate esters in the serum of young Puerto Rican girls with premature breast development”, Journal of Environmental Health Perspectives.pp.108895–900, 2001.
[8] C. FoliL. Haux, “Increased excretion in the bile of 17þ-[3H]- estradiol derived radioactivity in the rainbow trout treated with þ- naphthoflavone”, Aquat. Toxicology. 6-197–208, 1985.
[9] OS.Fatoki and F.Vernon, “Phthalate esters in rivers of greater Manchester area UK”, Sci. Total. Environ. 95, pp.227–232, 1980
[10] N.Ghorpade, V.Mehta, M.Khare, P.Sinkar, S. Krishnan and CV.Rao, “Toxicity study of diethyl phthalate on freshwater fish Cirrhinus mrigala”, Journal of Ecotoxicology and Environmental Safety. 53 255–258, 2002
[11] J Gartshore, J., Cooper, D.G., Nicell, J.A., 2003. Biodegradation of plasticizers by
Rhodotorularubra. Environmental toxicology and chemistry 22, 1244-1251, 2003
[12] CA.Giam, HS.Chan, GS. Nev and EL.Atlas, “ Phthalate ester plasticizers: a new class of marine pollutant”, Science, 199, pp.419–421, 1978
[13] S.Jobling, T. Reynolds, R.White, MG.Parker and JP.Sumpter, “A
variety of environmentally persistent chemicals, including some phthalate plasticizers are weakly estrogenic”, Environ. Health Perspect.103, pp.582–587, 1995
[14] Juhua Chang, Shaoying Liu, Shengli Zhou, Minghua Wang and Guonian Zhu, “Effects of butachlor on reproduction and hormone levels in adult zebrafish (Danio rerio)” Experimental and Toxicology and Pathology 65, pp.205–209, 2013
[15] AE. Karels, M.Soimasuo, J.Lappivaara, H. Leppanen, T.Aaltonen and Mellanen, “Effects of ECF-bleached kraft mill effluent on reproductive steroids
and liver MFO activity in populations of perch and roach”. Ecotoxicology;7, pp.123–32, 1998
[16] TH.Hutchinson, R.Brown, KE.Brugger, PM. Campbell, M.Holt and R.Lange, “Ecological risk assessment of endocrine disruptors”. Environmental Health perspective.108, pp.1007-1014, 2000
[17] A.Kermanshahi, DG.Cooper, OA.Mamer, M. Maric and JA.Nicell,
“Mechanisms of biodegradation of dibenzoate plasticizers”. Chemosphere,77, pp.258–263, 2009
[18] C.Mills L.Chichester, “Review of evidence: are endocrine-disrupting chemicals in the aquatic environment impacting fish populations”. Sci Total Environ 343, pp.1–34, 2005
[19] RT.Okita and JR.Okita, “Effects of diethyl phthalate and other plastic izersonlaurate hydroxylation in rat”, Pharma Res.9, pp.1648–1653, 1992
[20] PR.Rocha Monteiro, MA. Reis-Henriques and J.Coimbra, “Polycyclic aromatic hydrocarbons inhibit in vitro ovarian steroidogenesis in the
flounder (Platichthysflesus L.)”. Aquatic Toxicology.48, pp.549–59, 2000
[21] C.Sonneschein, AM.Soto, MF.Fernandez and N.Olea, “Development of a marker of estrogenic exposure in human serum”, Clin. Chem. 41, pp. 1888–1895, 1995
[22] H.Segner, “Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption”. Comp Biochem Physiol C 149,pp.187–95, 2009
[23] AM.Soto, C.Sonneschein, KL.Chung, MF.Fernandez, N. Olea and O.Serrano, “The E-screen assay as a tool to identify estrogen: an update on estrogenic environmental pollutants”, Environ. Health Perspect. 103, pp.13–122, 1995
[24] M.Sharman, WA.Read, L.Castle and J.Gilbert, “Levelsdi-{2-ethyl- hexyl} phthalate and total phthalate esters in milk, cream, butter and cheese”,Food Addit. Contam. 11, pp.375–385, 1994
[25] V.Sonde, A.Dsouza, R.Tarapore, L.Pereira, MP. Khare, P. Sinkar, S.Krishnan and CV. Rao, “Simultaneous administration of diethyl phtha- late and ethyl alcohol and its toxicity in male Sprague–Dawley rats”. Toxicology, 147, pp.23–31, 2000
[26] R.Thibaut. E.Perdu and JP. Cravedi, “ Testosterone metabolism as a possible biomarker of exposure off is htononyl phenol”, Rev.Me´d.Vet.153, (8–9), pp. 589–590, 2001
[27] K. Tollefsen, “Interaction of estrogen mimics, singly and in
combination, with plasma sex steroid-binding proteins in rainbow trout
(Oncorhynchusmykiss)” .Aquat. Toxicol.56, pp.215–25, 2002
[28] Z.Tong and MO.James, “Purification and characterization of hepatic and intestinal phenol sulfotransferase with high affinity for benzo[a]pyrene phenols from channel catfish, Ictaluruspunctatus,” Arch. Biochem. Biophys. 376(2) , pp. 409–419, 2000