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IJSTR >> Volume 8 - Issue 10, October 2019 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Degradation Of Polyphenol By Bacillus Subtilis Sma Isolated From Effluents Of Tanneries

[Full Text]



Dr. Salamun DE, Manesh Shah, Aishwarya Shetty



Polyphenols, Tanneries, polyphenol degrading microorganism, GenBank accession number: MK760577



The objective of the present study was to isolate and identify polyphenol degrading microorganism and to optimize the culture conditions for better yield and increased mass production. Waste water samples collected from the effluents of tanneries were filtered and serially diluted. The microbes were grown on nutrient agar medium for 24 hours. The isolated colonies were then transferred onto a mineral salt medium containing various concentration of polyphenol (tannic acid), followed by incubation at 370C for 72 hrs in CO2 incubator. The survival of microorganisms at various increasing concentration of polyphenol (50 ppm to 250 ppm) was performed. The plate which showed highest number of colonies was selected and the isolated colony was subjected to morphological, biochemical and molecular identification. The results showed that the isolate belonged to Bacillus genus and the resulting bacterial strain isolate was found to be Bacillus subtilis and the GenBank accession number was obtained which was MK760577. After identification, the culture conditions required for maximum enzyme production were optimized.



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