International Journal of Scientific & Technology Research

IJSTR@Facebook IJSTR@Twitter IJSTR@Linkedin
Home About Us Scope Editorial Board Blog/Latest News Contact Us

IJSTR >> Volume 4 - Issue 10, October 2015 Edition

International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616

Study On Callus Initiation And Plantlet Regeneration Ability Of Some Rice Genotypes

[Full Text]



Amrita Mukherjee, Mohd. Rezaul Islam Dr. Khondokar Mohammod Nasiruddin, Purba Banerjee



Index Terms: Rice genotypes, MS, NAA, IBA, Different concentration, Callus initiation and Plantlet Regeneration



Abstract: An experiment was conducted with mature embryos of three HYD and resistant rice (varieties BRRIdhan-29, BR-14, BINA dhan-8) as explants to investigate the appropriate growth condition for callus induction as well as plant regeneration on MS basal medium supplemented with different concentration of auxin and cytokinin. Different concentrations of 2, 4-D (0.0, 1.5, 2, 2.5, 3 mgL-1) were used for callus induction. Among the concentrations 2.0mgL-1 2, 4-D was best concentrations for callus induction (85%), callus size (4.533mm) and callus weight (81.847mg) in 10.66days and the highest maintaining ability was found in BR-14 at 2.0mgL-1 2, 4-D (88%) in callus size (5.143a) and callus weight (87.50mg)) in 6.50days. Different concentrations of kinetin (0.0, 0.4, 0.6, 0.8, 10 mgL-1) with a constant concentration of NAA (0.5mgL-1) were used for plant regeneration from callus. Again, BRRI dhan-29 on T3 (MS + 8 mgL-' Kn + 0.5 mgL-' NAA) was found to be best in shoot length (6.00cm) with highest shoots initiation (97.5%) and the highest .number of shoots per callus (5.25) on T2 (MS + 6 mgL-' Kn + 0.5 mgL-' NAA) in 22.25days. In combination, BRRI dhan-29 the highest number of roots per explant (7.25), root length (2.31cm) was found in T3 (MS + 0.6 mgL-1 IBA) and the highest root initiation (97.50%) was on T2 (MS + 0.5 mgL-1 IBA).



[1] Abbasi F.M.., Hamid R. and Azra Q. 2000. regeneration efficacy and embryogenic callus production of three cultivars of rice . Pakistan J.Agril. Res.16(2): 97-99.

[2] Al-khayri J.M. and Al-Bahrany A.M.2000.in vitro plant regeneration of Hassani Rice from Mature Embryo Callus .Pakistan J.Biol.Sci. 3(4): 602-605.

[3] Aktar, A. K., Zakria, M., Abbasi, F. M., and Masud, M. A. (2003). Incidence of Bacterial blight of rice in Bangladesh during 2002. Bangladesh J. Botany.35(5):993-996.

[4] Amin M.A., Uddin M.A. and Hossain M.A.2004.Regeneration study of some indica rice cultivars followed by Agrobacterium –mediated transformation of highly regenerable cultivar, BR-8.J.Biol.Sci.4(2):207-211.

[5] Asaduzzaman M., M.A., Rahman M.H. and Khatun N. 2003.In vitro plant regeneration through anther culture of five rice varieties , J.Biol.Sci.3(2): 167-171.

[6] Abe, T. and Futsuhara Y. 1984. Varietal differences in plant regeneration from root callus tissues in rice. Jpan J. Breed.34: 147-155.

[7] Aditya, T. L. and Baker , D.A. 2005. the effect of continious NaCl stress on the restoration of high callus induction frequency, regeneration potential and in vitro growth of somaclones of four Bangladeshi indica rice genotype s . Division of Plant Breeding, Bangladesh Rice Res. Ins ., Gazipur -1701, Bangladesh.42(2):103-108.

[8] Aditya , T.L.; Hoque, M. E. and Khalequzzaman , M. 2004. Response to high frequency callus Induction ability from root regions of germinated embryo in indica rice Pakistan J. of Biol.Sci. 7(5): 861-864.

[9] Aldemita , R. K. and Zapata , F.J.1996. Anther culture of rice. Effects of radiation and media Components on callus induction and plant regeneration. Cereal Res. Comm. 19(1-2): 9-32.

[10] Amarasinghe, A. A.Y. and Yang, Y. S.2005. Comparative Studies on In-Vitro Response of Fresh and Old Calli of Rice (Oryza sativa L.) The Journal of Agricultural Science, 2005, vol. 1, no.2.

[11] Anderson, E. J. and Al-Khayri, J. M. 1996. Effect of genotype and media component on induction and plant regeneration of rice. Res. Ser. Arkansas Agril. Exp. Tation. 453: 222-227.

[12] Afrasiab, H and Jafar, R.2011. Effect of different media and solidifying agents On callogenesis and plant regeneration from Different explants of rice (oryza sativa l)

[13] Varieties super basmati and irri-6. Pak. J. Bot., 43(1): 487-501, 2011.

[14] Azira, D and Bhalla , P.L.2000. Plant regeneration from mature embryo-derived callus of Australian rice (Oryza satia L.) varieties. Aust. J. Agril. Res. 51: 305-312.

[15] Bano, S.; Jabeen, M.; rahim , F and ILahi , .2005. Callus nduction and regeneration in seed Explants of rice (Oryza sativa cv. Swat-II). Pakistan J. Bot. 2005; 37(4): 829-836.

[16] Baset, A.; Tobita S.; LiC.; Yashima S.; Senboku T and R. A. Drew 1998. Micro propagation and in vitro culture of wild rice species , Proc. Int. Symp. Biotechnology of Tropical Species , Part nII, Bris bne , Queensland , Australia, 29 September 3 October. 1997. Acta. Horticulturae. 461: 259-266.

[17] BBS (Bangladesh Bureau of Statistics), 2006. monthly Statistically Bulletin of Bangladesh Bureau of Statistics. Statistics Division Ministry of Planning . Government of People’s Republic of Bangladesh. Pp: 54-58.

[18] Bhuyan, S.I. and Karim , A. N.M. R.2002. Rice production in Bangladesh : an overview . Bangladesh rice Research Institute . Joydebpur, Dhaka. Pp. 13-17.

[19] Brisibe, E.A.; and Mandal, A.B. 2006. Rapid and recurrent in vitro mass - multiplication of androgenic rice embryos. Indian J. Biotechnolo,5(2): 239-242.

[20] Bhaskaran S. and Smith R.H. 1988. Enhanced somatic embryogenesis in Oryza sativa L. from shoot tip culture . in vitro cell . Dev. Boil. Plant 24:65-70.

[21] Chang, T.T. 1964.present Knowledge of rice genetics and cytogenetics.IRRI Tech.Bull.1. 96pp.

[22] Chitra, S.; Kumar, C.R. A and Vivekanandan, P.2003. Somatic embryogenesis from indica rice coleoptiles. India J. Tiss. 11(2): 12-14.

[23] Datta, K.; Baisakh, N.; Oliva, N.; Torrizo , L.; and Datta, S.K.2003.Bioengineered “ golden” indica rice cultivar with beta caritene metabolism in the endosperm with hygrocin and mannose selection systems. J. Plant Biotechnolo. 1(2):81.

[24] Deepinder, G.; Raman , G and Gosal, S. S. 2005. Factors enhancing introduction of high frequency plant regeneration from somatic embryos of indica rice ?(Oryza sativa L.). India. J. Biol.Sci.5 (6): 697-702.

[25] Deepti S., Shankhdhar S.C., Plant R.C. and Shankhdhar D. 2001. Genotypic variation of callus induction and plant regeneration in rice (Oryza sativa L). Indian J. Plant Physiol.6 (3): 261-264

[26] Dodds, J.H. and Roberts, L. W. 1990. Anther and pollen culture. In: J. H. Dodds andL.W. Roberts (Eds), experiments in Plant Tissue Culture.Cambridge University Press. New York. Pp. 157-171.

[27] Doroseiv , l. 1986. Plant Cell and Tissue Cultures present stae and future prospects. Genetica is Selektsiya. 19(5)356-362.

[28] Duan, C.L.; Xiao, C. Rathus and Godwin, 2001. Establish of a tissue culture system for Agrobacterium mediated transformation of paddy rice. J. Southwest Gril. Uni. 23(6):557-531.
[29] Ezuka and kaku., 2000. A histrical review of bacterial blight of rice.Department of genetic resource II and I. Bull. Natl. Inst. Agrobiol. Rsource 15: 1-207.

[30] Eamchit, S. and Mew, T.W. 1982.” Plant Diseases” An International Journal Of Applied Plant Pathology. vol.66. No. 7

[31] FAO (Food and Agriculture Organization) 2002. Production Year Book. Food and Agriculture Organization of the United Nation. Rome 57: 76-77.

[32] FAO (Food and Agriculture Organization) 2004. Rice is life. Italy: FAO. Http://www.fao.org/newsroom/en/focus/20436887/index.html.

[33] Feng S.H.; Li. Y.S. and Li, Y S.995. effects of agar concentration in media on green plantlet regeneration frequency in rice anther culture. CRRN Chinese Rice Research Newsletter.7(2):7-8.

[34] Fujiwara, A. and Ojima , K. 1955. principles and practices rice production .Jpan, kagaku 34, 623.

[35] Ge, X.; Chu, Z.; Lin, Y. and Wang, S. 2006. A tissue culture system for different germplasms of indica rice. Plant Cell rep. 25(5): 392-402.

[36] Gonzalez, M.C.2000.Effect of different growth regulators on in vitro culture of ice cv. Amistad-82. cultivos- tropicales.21(1):27-28.
[37] Gandhi R. and Khurana P. 1999. Stress mediated regeneration from mature embryo deried calli and gene transfer through Agrobacterium in rice (Oryza sativa ). Indian Exp. Biol.37(4): 332-339.

[38] Gomez S.M. and Kalamani A. 2002. Varibility analysis of traits related to callus growth and plant regeneration in drought ressitant local land races of rice (Oryza sativa L.). Asaian J. Plant Sci. 1(5): 583-584.

[39] Gould,J. 1996. Transformation of the cereals using Agrobacterium . In moleculer biology. Vol.62Ed.Recombination Gene Expression Protocols. R. Tuan human Inc. Totawa, Newjerey , pp.491-501.

[40] Guo, C.Y. and Cao , Z.Y.1982. Effect of different genotypes on induction frequency in anther and scutellum culture of maize in vitro.Heredities , china . 4(4):8-10.

[41] He,T.; Yang, Y.; Tu, S.B.2006. Selection of interspecific hybrids for anther culture of indica rice . Plant Cell Tiss. Org.Cult.86(2): 271-277.