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IJSTR >> Volume 7 - Issue 3, March 2018 Edition



International Journal of Scientific & Technology Research  
International Journal of Scientific & Technology Research

Website: http://www.ijstr.org

ISSN 2277-8616



Molecular Epidemiology Of Peste Des Petits Ruminants (PPR) In Goat

[Full Text]

 

AUTHOR(S)

Md. Raihan Nabi, Md. Shahadat Hossain, Sukumar Saha, Jahangir Alam, Md. Giasuddin

 

KEYWORDS

Peste des petits ruminants (PPR); Molecular Epidemiology; RT-PCR; F gene sequencing.

 

ABSTRACT

The study was planned to carry out the molecular epidemiology of PPR. The data (n=143) were collected from two village goat and a market of Shibgonj, Bogra, Bangladesh and nasal swabs (n=72) were collected from clinically suspected goats from twelve Veterinary Hospitals of seven districts during July-October 2012. The virus was detected by RT-PCR technique in which PPRV: F1b & F2d; NP3 & NP4 primers were used to detect and F gene of three isolates (Named PPRV/BD/Rajshahi-1/2012, PPRV/BD/Rajshahi-2/2012 and PPRV/BD/Bogra/2012) were sequenced and phylogenetic analyses were performed. The survey resulted that the overall morbidity and mortality were 19.79 and 11.51 % respectively in the post outbreak period as well as the molecular study confirmed that 33.33 % samples were diagnosed as positive and this can be interpreted as, the goat were suffering from high fever with or without diarrhea still incubating the disease, the highest morbidity and mortality were found in male goats and in goats of age between 4-12 months. The N gene was revealed 100% confirmatory diagnosis in comparison with F. The findings of the nucleotide sequences of F gene fragments revealed that each of the isolates belonged to Lineage IV and most closely related to the others obtained from Gene Bank. PPRV/BD/Bogra/2012 has 100% homology in terms of partial sequence, 289bp of F gene with the viruses- Narayangonj/2009(Ac.No.JX094440), Dhaka/2009(Ac.No.JX094438) and BAU/Mymensingh/2010 (Ac.No.JX094436).

 

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