The Effects Of Plant Growth Regulators (Naa+Bap) And Explant Types On Propagation Buds Of Asam Gelugur (Garcinia Atroviridis Griff)
[Full Text]
AUTHOR(S)
Nurcholis Alfarisi, Luthfi Aziz Mahmud Siregar, Tengku Chairun Nisa
KEYWORDS
apical shoot, axillary bud, BAP, Garcinia atroviridis, NAA.
ABSTRACT
The research was aimed to determine the effect rasio combination of Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) plant growth regulators and explant types of Asam Gelugur plant (Garcinia atroviridis Griff) and their interaction in the Murashige Skoog (MS) media. This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Sumatra Utara, Indonesia in February until June 2018. The research was used the completely randomized design with 2 treatment factors with three replications. The first factors is combination of NAA and BAP dosage with nine rates, including G1 (0.2 mg.l-1 NAA + 0.5 mg.l-1 BAP); G2 (0.2 mg.l-1 NAA + 1 mg.l-1 BAP); G3 (0.2 mg.l-1 NAA + 1.5 mg.l-1 BAP); G4 (0.4 mg.l-1 NAA + 0.5 mg.l-1 BAP); G5 (0.4 mg.l-1 NAA + 1 mg.l-1 BAP); G6 (0.4 mg.l-1 NAA + 1.5 mg.l-1 BAP); G7 (0.6 mg.l-1 NAA + 0.5 mg.l-1 BAP); G8 (0.6 mg.l-1 NAA + 1 mg.l-1 BAP); and G9 (0.6 mg.l-1 NAA + 1.5 mg.l-1 BAP). The second factors is the explant types such as apical shoot (E1) and axillary bud (E2). The results were showed that the addition of 0.2 mg.l-1 NAA + 1 mg.l-1 BAP (G2) in the MS media had significant effect on the percentage of bud formed, number of buds, length of buds, and number of leaves. The axillary bud explant (E2) had significant on the number of buds. The interaction of E2G2 had significant on the number of
REFERENCES
[1] Uji, T. “Diversity, distribution and potential of genus Garcinia in Indonesia”, Hayati, 12: 129-135, 2007.
[2] Amran, A. A., Zaiton, Z., Faizah, O., and Paden, M., ”Effect of Garcinia atroviridis on oxidative stress and atherosclerotic changes in experimental guinea pigs. American Journal of Pharmacology and Toxicology, 5(2): 65-70, 2010.
[3] Mackeen, M. M., Ali, A. M., Lajis, N. H., Kawazu, K., Hassan, Z., Amran, M., Habsah, M., Mooi, L.Y., and Mohamed, S.M., “Antimicrobial, antioxidant, antitumour-promoting and cytotoxic activities of different plant part extracts of Garcinia atroviridis Griff. ex T. Anders”, Journal of Ethnopharmacology, 72(3): 395-402, 2000. https://doi.org/10.1016/S0378-8741(00)00245-2.
[4] Chung, C. S., “Sweet and sour, the lovely gelugor”, Gardenwise, 2(6): 18-19, 2006.
[5] George, E. F., and Sherington, P. D., “Plant propagation by tissue culture’, Handbook and Directory of Commercial Laboratories. Exegetic. England, l984.
[6] Yusnita, “Kultur jaringan: cara memperbanyak tanaman secara efisien”, PT Agromedia Pustaka, Jakarta, 2003.
[7] Wattimena, G. A., “Zat pengatur tumbuh tanaman”, Laboratorium Kultur Jaringan Tanaman PAU Bioteknologi IPB. Bogor. 145 hlm, 1988.
[8] Gardner, F. P., Pearce, R. B., and Mitchell, R. L., “Fisiologi Tanaman Budidaya”, Universitas Indonesia Press. Jakarta, 1991.
[9] Santoso, U., and Nursandi, F., “Kultur jaringan tanaman”, Penerbit UMM, Malang, 2001.
[10] Astuti., and Andayani., “Pengaruh pemberian BAP dan NAA terhadap pertumbuhan krisan (Chysanthemum morifoluim, Ram). Jurnal Kultur Jaringan Biota X, 3: 31-35, 2005.
[11] Dwiyani, R., “Kultur jaringan tanaman”, Pelawa Sari, 75: 65-66, 2015.
[12] Anitasari, S. D., Sari, D. R., Astarini, I. A., and Defiani, M. R., “Dasar Teknik Kultur Jaringan”, CV Budi Utama, Yogyakarta, 2018.
[13] Murashige, T., and Skoog, F., “A revised medium for rapid growth and bio assays with tobacco tissue cultures”, Physiologia plantarum, 15(3): 473-497, 1962. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[14] Tabiyeh, D. T., Bernard, F., and Shacker, H., “Investigation of glutathione, salicylic acid and GA3 effects on browning in Pistacia vera shoot tips culture”, In IV International Symposium on Pistachios and Almonds, 726: 201-204, 2005. https://doi.org/10.17660/ActaHortic.2006.726.31.
[15] Harahap, P. S., Siregar, L. A. M., and Husni, Y., “Kajian awal : Respon eksplan nodus dalam inisiasi tunas mikro tanaman karet (Hevea brasiliensis muell Arg.) dalam medium MS”, Jurnal Online Agroekoteknologi, 3(1): 229–237, 2014.
[16] Sundari, L., Siregar, L. A. M., and Hanafiah, D. S., “Kajian awal: respon eksplan nodus dalam inisiasi tunas mikro tanaman karet (Hevea brasiliensis Muell. Arg.) dalam medium WPM”, Jurnal Online Agroekoteknologi, 3(1): 179-187, 2015.
[17] Wetherell, D. F., “Pengantar propagasi tanaman secara in vitro”, Terjemahan : Koensumardiyah. Avery Publishing Group Inc., Wayne, New Jersey, 1982.
[18] Fereol, L., Chovelon, V., Causse, S., Michaux-Ferriere, N., and Kahane, R., “Evidence of a somatic embryogenesis process for plant regeneration in garlic (Allium sativum L.)”, Plant Cell Reports, 21(3): 197-203, 2002. https://doi.org/10.1007/s00299-002-0498-0.
[19] Widyastuti, N., and Deviyanti, J., “Kultur jaringan, teori dan praktik perbanyakan tanaman secara in vitro”, Andi, Yogyakarta, 2018.
[20] Karjadi, A. K., and Buchory, A., “Pengaruh NAA dan BAP terhadap pertumbuhan jaringan meristem bawang putih pada media B5”, Jurnal Hortikultura, 17(3): 217-223, 2007. http://dx.doi.org/10.21082/jhort.v17n3.2007.p%25p.
[21] Salisbury, F. B., and Ross, C. W., ”Fisiologi tumbuhan, biokimia tumbuhan Jilid 2”, Penerjemah: Lukman, DR dan Sumaryono. Bandung: Penerbit ITB, 1995.
[22] Anggraini, D., “Pengaruh konsentrasi indole 3-butiryc acid (IBA) dan 6- benzylaminopurine (BAP) terhadap pertumbuhan tanaman anthurium (Anthurium plowmanii Croat.)”, Skripsi, Universitas Sebelas Maret, Surakarta, 2007.
[23] Widyastuti, K., “Pengaruh kombinasi NAA dan BAP terhadap induksi tunas aksilar tanaman balsam (Poligala paniculata L) secara In vitro”, Skripsi, Universitas Islam Negeri Maulana Malik Ibrahim. Malang, 2016.
|